Precise, wide field, and low-cost imaging and analysis of core–shell beads for digital polymerase chain reaction

Ajeet Singh Yadav, Fariba Malekpour Galogahi, Du Tuan Tran, Aditya Vashi, Chin Hong Ooi, Kamalalayam Rajan Sreejith and Nam-Trung Nguyen

Lab Chip, 2023, 23, 3353
DOI: 10.1039/D3LC00337J

A handheld flow genetic analysis system (FGAS): towards rapid, sensitive, quantitative and multiplex molecular diagnosis at the point-of-care level

Bowen Shu, Chunsun Zhang and Da Xing

Lab Chip, 2015, 15, 2597
DOI: 10.1039/C5LC00139K

A sample-to-answer DNA detection microfluidic system integrating sample pretreatment and smartphone-readable gradient plasmonic photothermal continuous-flow PCR

Zengming Zhang, Shuhao Zhao, Lei Jiang, Junjun Wu, Wenhan Zhao, Xiaoniu Guo, Niancai Peng and Fei Hu

Analyst, 2022, 147, 4876
DOI: 10.1039/D2AN00908K

Diameter-definable tubing-microchips for applications in both continuous-flow and TEC-modulated on-chip qPCRs with reaction signal analyzed between different types of Teflon-polymers: PTFE and FEP

Yangyang Jiang, Guizhu Wu, Yuanming Li and Wenming Wu

RSC Adv., 2019, 9, 2650
DOI: 10.1039/C8RA09773A

Heat and pressure-resistant room temperature irreversible sealing of hybrid PDMS–thermoplastic microfluidic devices via carbon–nitrogen covalent bonding and its application in a continuous-flow polymerase chain reaction

Rajamanickam Sivakumar, Kieu The Loan Trinh and Nae Yoon Lee

RSC Adv., 2020, 10, 16502
DOI: 10.1039/D0RA02332A

A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor

Bing Shi, Yuanming Li, Di Wu and Wenming Wu

Analyst, 2020, 145, 2767
DOI: 10.1039/C9AN01894H

A novel mechanism for user-friendly and self-activated microdroplet generation capable of programmable control

Yangyang Jiang, Lin Du, Yuanming Li, Quanquan Mu, Zhongxu Cui, Jia Zhou and Wenming Wu

Analyst, 2018, 143, 3798
DOI: 10.1039/C8AN00035B

Capillary-based integrated digital PCR in picoliter droplets

Jinyu Chen, Zhaofeng Luo, Lin Li, Jinlong He, Luoquan Li, Jianwei Zhu, Ping Wu and Liqun He

Lab Chip, 2018, 18, 412
DOI: 10.1039/C7LC01160A

Miniaturized Electrically Actuated Microfluidic System for Biosensor Applications

Salvatore Petralia, Maria Eloisa Castagna, Daniele Motta and Sabrina Conoci

BioNanoSci., 2016, 6, 139
DOI: 10.1007/s12668-016-0204-2

Thermal cycling characteristics of a 3D-printed serpentine microchannel for DNA amplification by polymerase chain reaction

Jaehyun Park and Heesung Park

Sensors and Actuators A: Physical, 2017, 268, 183
DOI: 10.1016/j.sna.2017.10.044

Nucleic Acids Analysis

Yongxi Zhao, Xiaolei Zuo, Qian Li, Feng Chen, Yan-Ru Chen, Jinqi Deng, Da Han, Changlong Hao, Fujian Huang, Yanyi Huang, Guoliang Ke, Hua Kuang, Fan Li, Jiang Li, Min Li, Na Li, Zhenyu Lin, Dingbin Liu, Juewen Liu, Libing Liu, Xiaoguo Liu, Chunhua Lu, Fang Luo, Xiuhai Mao, Jiashu Sun, Bo Tang, Fei Wang, Jianbin Wang, Lihua Wang, Shu Wang, Lingling Wu, Zai-Sheng Wu, Fan Xia, Chuanlai Xu, Yang Yang, Bi-Feng Yuan, Quan Yuan, Chao Zhang, Zhi Zhu, Chaoyong Yang, Xiao-Bing Zhang, Huanghao Yang, Weihong Tan and Chunhai Fan

Sci. China Chem., 2021, 64, 171
DOI: 10.1007/s11426-020-9864-7

Molecular-biological sensing in aquatic environments: recent developments and emerging capabilities

Jonathan S McQuillan and Julie C Robidart

Current Opinion in Biotechnology, 2017, 45, 43
DOI: 10.1016/j.copbio.2016.11.022

Advance in Research of Microfluidic Polymerase Chain Reaction Chip

Qi-Di HE, Dan-Ping HUANG, Guan HUANG and Zuan-Guang CHEN

Chinese Journal of Analytical Chemistry, 2016, 44, 542
DOI: 10.1016/S1872-2040(16)60921-0

Miniaturization of fluorescence sensing in optofluidic devices

Daniel Măriuţa, Stéphane Colin, Christine Barrot-Lattes, Stéphane Le Calvé, Jan G. Korvink, Lucien Baldas and Jürgen J. Brandner

Microfluid Nanofluid, 2020, 24
DOI: 10.1007/s10404-020-02371-1

Thermal study of polymerase chain reaction with capillary tubes

Hassan Ali Abid, Jian Wern Ong, Zhixiong Song, Eric Shen Lin, Oi Wah Liew and Tuck Wah Ng

International Journal of Heat and Mass Transfer, 2021, 176, 121508
DOI: 10.1016/j.ijheatmasstransfer.2021.121508

Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

Maria Serena Chiriacò, Monica Bianco, Annamaria Nigro, Elisabetta Primiceri, Francesco Ferrara, Alessandro Romano, Angelo Quattrini, Roberto Furlan, Valentina Arima and Giuseppe Maruccio

Sensors, 2018, 18, 3175
DOI: 10.3390/s18103175

Emerging Droplet Microfluidics

Luoran Shang, Yao Cheng and Yuanjin Zhao

Chem. Rev., 2017, 117, 7964
DOI: 10.1021/acs.chemrev.6b00848

Observability‐Based Sensor Placement Improves Contaminant Tracing in River Networks

Matthew Bartos and Branko Kerkez

Water Resources Research, 2021, 57
DOI: 10.1029/2020WR029551

Closed-Loop Microreactor on PCB for Ultra-Fast DNA Amplification: Design and Thermal Validation

Panagiotis Skaltsounis, George Kokkoris, Theodoros G. Papaioannou and Angeliki Tserepi

Micromachines, 2023, 14, 172
DOI: 10.3390/mi14010172

A BODIPY‐Based Donor/Donor–Acceptor System: Towards Highly Efficient Long‐Wavelength‐Excitable Near‐IR Polymer Dots with Narrow and Strong Absorption Features

Lei Chen, Dandan Chen, Yifei Jiang, Jicheng Zhang, Jiangbo Yu, Christopher C. DuFort, Sunil R. Hingorani, Xuanjun Zhang, Changfeng Wu and Daniel T. Chiu

Angewandte Chemie, 2019, 131, 7082
DOI: 10.1002/ange.201902077

A Microfluidic Approach for Biosensing DNA within Forensics

Brigitte Bruijns, Roald Tiggelaar and Han Gardeniers

Applied Sciences, 2020, 10, 7067
DOI: 10.3390/app10207067

Continuous‐Flow Microfluidic Device for Real‐Time Polymerase Chain Reaction

Hanok Kim, Shinae Suk, Kwanseop Lim, Nokyoung Park and Jong Hoon Hahn

Bulletin Korean Chem Soc, 2016, 37, 1878
DOI: 10.1002/bkcs.10982

A digital PCR system based on the thermal cycled chip with multi helix winding capillary

Bin Li, Yuanming Li, Yangyang Jiang, Andreas Manz and Wenming Wu

Sci Rep, 2020, 10
DOI: 10.1038/s41598-020-74711-8

Plug-in optical fiber SPR biosensor for lung cancer gene detection with temperature and pH compensation

Xuegang Li, Pengqi Gong, Qiming Zhao, Xue Zhou, Yanan Zhang and Yong Zhao

Sensors and Actuators B: Chemical, 2022, 359, 131596
DOI: 10.1016/j.snb.2022.131596

Digital polymerase chain reaction duplexing method in a single fluorescence channel

Haoqing Zhang, Soňa Laššáková, Zhiqiang Yan, Xinlu Wang, Pavel Šenkyřík, Martina Gaňová, Honglong Chang, Marie Korabečná and Pavel Neuzil

Analytica Chimica Acta, 2023, 1238, 340243
DOI: 10.1016/j.aca.2022.340243

Development of small-sized fluorescence detector for pipette tip-based biosensor for on-site diagnosis

Masakazu Kagawa, Kazuhiro Morioka, Moeko Osashima, Akihide Hemmi, Shoji Yamamoto, Atsushi Shoji, Katsumi Uchiyama and Hizuru Nakajima

Talanta, 2023, 256, 124311
DOI: 10.1016/j.talanta.2023.124311

Consecutive and automatic detection of multi-gene mutations from colorectal cancer samples by coupling droplet array-based capillary electrophoresis and PCR-RFLP

Yiming Feng, Tingting Hu, Pan Fang, Linlin Zhou, Wanming Li, Qun Fang and Jin Fang

Anal Bioanal Chem, 2020, 412, 3037
DOI: 10.1007/s00216-020-02567-y

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

Min-Sheng Hung, Chia-Chin Ho and Chih-Pin Chen

J. Biomed. Opt, 2016, 21, 087003
DOI: 10.1117/1.JBO.21.8.087003

Miniaturized Continuous-Flow Digital PCR for Clinical-Level Serum Sample Based on the 3D Microfluidics and CMOS Imaging Device

Bin Li, Yuanming Li, Andreas Manz and Wenming Wu

Sensors, 2020, 20, 2492
DOI: 10.3390/s20092492

A review on microscale polymerase chain reaction based methods in molecular diagnosis, and future prospects for the fabrication of fully integrated portable biomedical devices

Nae Yoon Lee

Microchim Acta, 2018, 185
DOI: 10.1007/s00604-018-2791-9

Compressed Air-Driven Continuous-Flow Thermocycled Digital PCR for HBV Diagnosis in Clinical-Level Serum Sample Based on Single Hot Plate

Kangning Wang, Bin Li and Wenming Wu

Molecules, 2020, 25, 5646
DOI: 10.3390/molecules25235646

PID Temperature Control System-Based Microfluidic PCR Chip for Genetic Analysis

Hyo Eun Kim, Ariadna Schuck, Won-Young Kim, Eun Kyo Jung, Yong-Hoo Hong and Yong-Sang Kim

J. Electr. Eng. Technol., 2022, 17, 495
DOI: 10.1007/s42835-021-00969-1

A review on continuous-flow microfluidic PCR in droplets: Advances, challenges and future

Yonghao Zhang and Hui-Rong Jiang

Analytica Chimica Acta, 2016, 914, 7
DOI: 10.1016/j.aca.2016.02.006

A BODIPY‐Based Donor/Donor–Acceptor System: Towards Highly Efficient Long‐Wavelength‐Excitable Near‐IR Polymer Dots with Narrow and Strong Absorption Features

Lei Chen, Dandan Chen, Yifei Jiang, Jicheng Zhang, Jiangbo Yu, Christopher C. DuFort, Sunil R. Hingorani, Xuanjun Zhang, Changfeng Wu and Daniel T. Chiu

Angew Chem Int Ed, 2019, 58, 7008
DOI: 10.1002/anie.201902077

Droplet microfluidic platform for fast and continuous-flow RT-qPCR analysis devoted to cancer diagnosis application

I. Hajji, M. Serra, L. Geremie, I. Ferrante, R. Renault, J.-L. Viovy, S. Descroix and D. Ferraro

Sensors and Actuators B: Chemical, 2020, 303, 127171
DOI: 10.1016/j.snb.2019.127171

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

Jingwen Wang, Han Jiang, Leiming Pan, Xiuying Gu, Chaogeng Xiao, Pengpeng Liu, Yulong Tang, Jiehong Fang, Xiaoqian Li and Chenze Lu

Front. Bioeng. Biotechnol., 2023, 11
DOI: 10.3389/fbioe.2023.1020430

Seeking Solutions for Inclusively Economic, Rapid, and Safe Molecular Detection of Respiratory Infectious Diseases: Comprehensive Review from Polymerase Chain Reaction Techniques to Amplification-Free Biosensing

Yaping Xie, Zisheng Zong, Qin Jiang, Xingxing Ke and Zhigang Wu

Micromachines, 2025, 16, 472
DOI: 10.3390/mi16040472

Microfluidic Devices for Forensic DNA Analysis: A Review

Brigitte Bruijns, Arian Van Asten, Roald Tiggelaar and Han Gardeniers

Biosensors, 2016, 6, 41
DOI: 10.3390/bios6030041

A State‐Of‐The‐Art Review of Aquatic eDNA Sampling Technologies and Instrumentation: Advancements, Challenges, and Future Prospects

Kevan M. Yamahara, Elizabeth A. Allan, Julie Robidart, William H. Wilson, James M. Birch, Pascal Craw, Ethan Edson, Ivory B. Engstrom, Tatsuhiro Fukuba, Annette F. Govindarajan, Alfredo M. Martins, Kim M. Parsons, Vincent J. Sieben, Austen Thomas, Ian Wilson and Christopher A. Scholin

Environmental DNA, 2025, 7
DOI: 10.1002/edn3.70170