Recent advances in stimuli-responsive polymeric carriers for controllable CRISPR/Cas9 gene editing system delivery

Panqin Ma; Qi Wang; Xi Luo; Liuzhou Mao; Zhanxiang Wang; Enyi Ye; Xian Jun Loh; Zibiao Li; Yun-Long Wu

doi:10.1039/D3BM00529A

Recent advances in stimuli-responsive polymeric carriers for controllable CRISPR/Cas9 gene editing system delivery

Panqin Ma,†^a Qi Wang,†^a Xi Luo,†^b Liuzhou Mao,^a Zhanxiang Wang,*^b Enyi Ye,

^cd Xian Jun Loh,

^cd Zibiao Li

*^cde and Yun-Long Wu

*^a

Author affiliations

* Corresponding authors

^a Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China
E-mail: wuyl@xmu.edu.cn

^b BE/Phase I Clinical Center, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen 361000, China
E-mail: wangzx@xmu.edu.cn

^c Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 2 Fusionopolis Way, Innovis, #08-03, Singapore 138634, Singapore

^d Institute of Sustainability for Chemicals, Energy and Environment (ISCE2), A*STAR, 1 Pesek Road, Jurong Island, Singapore

^e Department of Materials Science and Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore
E-mail: lizb@imre.a-star.edu.sg

Abstract

Non-viral polymeric vectors with good biocompatibility have been recently explored as delivery systems for clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) nucleases. In this review, based on current limitations and critical barriers, we summarize the advantages of stimulus-responsive polymeric delivery vectors (i.e., pH, redox, or enzymes) towards controllable CRISPR/Cas9 genome editing system delivery as well as the advances in using stimulus-responsive CRISPR/Cas9 polymeric carriers towards cancer treatment. Last but not least, the key challenges and promising development strategies of stimulus-responsive polymeric vector designs for CRISPR/Cas9 systems will also be discussed.

This article is part of the themed collection: CRISPR Biomaterials

Article information

https://doi.org/10.1039/D3BM00529A

Article type

Review Article

Submitted

29 Cig 2023

Accepted

17 Cax 2023

First published

19 Cax 2023

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Biomater. Sci., 2023,11, 5078-5094

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Recent advances in stimuli-responsive polymeric carriers for controllable CRISPR/Cas9 gene editing system delivery

P. Ma, Q. Wang, X. Luo, L. Mao, Z. Wang, E. Ye, X. J. Loh, Z. Li and Y. Wu, Biomater. Sci., 2023, 11, 5078 DOI: 10.1039/D3BM00529A

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Biomaterials Science

Recent advances in stimuli-responsive polymeric carriers for controllable CRISPR/Cas9 gene editing system delivery

Abstract

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Recent advances in stimuli-responsive polymeric carriers for controllable CRISPR/Cas9 gene editing system delivery

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