Issue 24, 2019

Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Abstract

Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.

Graphical abstract: Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Supplementary files

Article information

Article type
Communication
Submitted
10 Cax 2019
Accepted
29 Cax 2019
First published
30 Cax 2019

Org. Biomol. Chem., 2019,17, 5920-5924

Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

K. Huang, F. Parmeggiani, H. Ledru, K. Hollingsworth, J. Mas Pons, A. Marchesi, P. Both, A. P. Mattey, E. Pallister, G. S. Bulmer, J. M. van Munster, W. B. Turnbull, M. C. Galan and S. L. Flitsch, Org. Biomol. Chem., 2019, 17, 5920 DOI: 10.1039/C9OB01089K

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