Ultrasensitive ELISA+ enhanced by dendritic mesoporous silica nanoparticles

Chang Lei; Chun Xu; Amanda Nouwens; Chengzhong Yu

doi:10.1039/C6TB01023G

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Ultrasensitive ELISA⁺ enhanced by dendritic mesoporous silica nanoparticles†

Chang Lei,^a Chun Xu,^a Amanda Nouwens^ab and Chengzhong Yu*^a

Author affiliations

* Corresponding authors

^a Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia
E-mail: c.yu@uq.edu.au

^b School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia

Abstract

An ultrasensitive enzyme-linked immunosorbent assay (ELISA⁺) has been developed by using dendritic mesoporous silica nanoparticles (DMSNs) with high horseradish peroxidase (HRP) loading and easily accessible pore channels for signal amplification. A 2000 times enhancement of detection sensitivity compared to a commercial ELISA kit for insulin detection in serum is achieved.

This article is part of the themed collection: 2016 Journal of Materials Chemistry B Hot Papers

Download options Please wait...

Supplementary files

Supplementary information PDF (934K)

Article information

DOI: https://doi.org/10.1039/C6TB01023G
Article type: Communication
Submitted: 24 Agd 2016
Accepted: 27 Qas 2016
First published: 28 Qas 2016

Download Citation

J. Mater. Chem. B, 2016,4, 4975-4979

Permissions

Request permissions

Ultrasensitive ELISA⁺ enhanced by dendritic mesoporous silica nanoparticles

C. Lei, C. Xu, A. Nouwens and C. Yu, J. Mater. Chem. B, 2016, 4, 4975 DOI: 10.1039/C6TB01023G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Journal of Materials Chemistry B