Issue 20, 2018

Multidrug-eluting bi-layered microparticle-mesh scaffolds for musculoskeletal tissue regeneration

Abstract

Stem cell-based tissue engineering necessitates the development of a biocompatible scaffold, as a structural support, that provides a continuous supply of bioactive molecules for specific lineage differentiation. While incorporating bioactive molecules within a scaffold to improve stem cell differentiation has been reported in the literature, there is minimal evidence of any scaffold that can deliver a customized concoction of both hydrophobic and hydrophilic bioactive molecules to induce in situ lineage differentiation without any external supplements. In this study, we established a bioactive, drug-eluting bi-layered microparticle-mesh scaffold (BMMS) using the electrospinning technique. This BMMS was co-encapsulated with hydrophobic dexamethasone (in the mesh), hydrophilic ascorbic acid and β-glycerophosphate or proline (in the microparticles). We hypothesized that a sustained-releasing BMMS can direct in situ specific lineage differentiation of MSCs (e.g. osteogenic and chondrogenic) in a minimally supplemented culture environment into musculoskeletal tissues. The characterization of this BMMS revealed good encapsulation efficiencies of the bioactive molecules with sustained-releasing capabilities. The release kinetics of each drug was further analyzed using mathematical drug-releasing models. These scaffolds were subsequently shown to have potential for osteogenic or chondrogenic lineage differentiation from mesenchymal stem cells (MSCs) in a minimally supplemented culture medium.

Graphical abstract: Multidrug-eluting bi-layered microparticle-mesh scaffolds for musculoskeletal tissue regeneration

Supplementary files

Article information

Article type
Paper
Submitted
09 Nah 2018
Accepted
24 Agd 2018
First published
01 Cax 2018

J. Mater. Chem. B, 2018,6, 3340-3347

Multidrug-eluting bi-layered microparticle-mesh scaffolds for musculoskeletal tissue regeneration

V. N. Chamundeswari, Y. J. Chuah and S. C. J. Loo, J. Mater. Chem. B, 2018, 6, 3340 DOI: 10.1039/C8TB00397A

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