Issue 2, 2016

New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

Abstract

Covering: 2010 to 2015

Natural product scaffolds remain a major source and inspiration for human therapeutics. However, generation of a natural product in the post-genomic era often requires reconstruction of the corresponding biosynthetic gene cluster in a heterologous host. In the burgeoning fields of synthetic biology and metabolic engineering, a significant amount of efforts has been devoted to develop DNA assembly techniques with higher efficiency, fidelity, and modularity, and heterologous expression systems with higher productivity and yield. Here we describe recent advances in DNA assembly and host engineering and highlight their applications in natural product discovery and engineering.

Graphical abstract: New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

Article information

Article type
Highlight
Submitted
02 Leq 2015
First published
09 Kax 2015

Nat. Prod. Rep., 2016,33, 174-182

Author version available

New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

Y. Luo, B. Enghiad and H. Zhao, Nat. Prod. Rep., 2016, 33, 174 DOI: 10.1039/C5NP00085H

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