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Abstract | Cited by

A site-specific dual-color labeled ubiquitin for sensing deubiquitinase's activity was prepared by consecutively using chemoselective native chemical ligation reactions in a facile and efficient way. The prepared sensor was applied to establish a sensitive FRET-based assay for UCH-L3.

Graphical abstract: Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

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