Issue 8, 2014

Fast analysis of β-ecdysone in Brazilian ginseng (Pfaffia glomerata) extracts by high-performance liquid chromatography using a fused-core column

Abstract

The recent development of fused-core technology in HPLC columns is enabling faster and highly efficient separations. This technology was evaluated for the development of a fast analysis method for β-ecdysone in extracts of Pfaffia glomerata. A step-by-step strategy was used to optimize temperature (30–55 °C), flow rate (1.0–2.0 mL min−1), mobile phase composition (mixtures of water and methanol or acetonitrile) and equilibration time (1–5 min). A gradient method has been developed using two solvents: 0.1% acetic acid in water and 0.1% acetic acid in acetonitrile. Optimized conditions provided a method for the separation of β-ecdysone in approximately 2 min with a total analysis time (sample-to-sample) of 9 min, including the return to initial conditions and the re-equilibration of the column. Evaluation of chromatographic performance revealed excellent intraday and interday reproducibility (>99.5%), resolution (2.78), selectivity (1.13), and peak symmetry (1.09) while presenting low limits of detection (0.20 mg L−1) and quantitation (0.67 mg L−1). The robustness of the method has also been calculated according to the concentration/dilution of the sample. Several sample solvents were evaluated and the best chromatographic results were obtained using 80% methanol in water. Finally, the developed method was validated with different extracts of Pfaffia glomerata samples.

Graphical abstract: Fast analysis of β-ecdysone in Brazilian ginseng (Pfaffia glomerata) extracts by high-performance liquid chromatography using a fused-core column

Article information

Article type
Paper
Submitted
19 Kax 2013
Accepted
31 Qun 2014
First published
03 Nah 2014

Anal. Methods, 2014,6, 2452-2459

Author version available

Fast analysis of β-ecdysone in Brazilian ginseng (Pfaffia glomerata) extracts by high-performance liquid chromatography using a fused-core column

M. A. Rostagno, I. C. N. Debien, R. Vardanega, G. C. Nogueira, G. F. Barbero and M. A. A. Meireles, Anal. Methods, 2014, 6, 2452 DOI: 10.1039/C3AY42276C

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