Issue 6, 2018

A mitochondria-targeted fluorescent probe for selective detection of mitochondrial labile Fe(ii)

Abstract

Mitochondria are iron-rich organelles that are involved in the process of energy production through the electron-transporting system and heme synthesis. We developed a new mitochondria-targeted fluorescent probe, MtFluNox/Ac-MtFluNox, for Fe(II) based on N-oxide chemistry, which we recently established as a Fe(II)-selective fluorogenic switch. The deacetylated form MtFluNox showed a turn-on response towards Fe(II) with high metal selectivity in cuvette experiments, and an imaging study using its cell-compatible analogue Ac-MtFluNox demonstrated mitochondria-specific fluorescence enhancement in response to Fe(II) in living cells. Furthermore, the probe was able to detect endogenously accumulated Fe(II) induced as a result of the inhibition of heme synthesis.

Graphical abstract: A mitochondria-targeted fluorescent probe for selective detection of mitochondrial labile Fe(ii)

Supplementary files

Article information

Article type
Paper
Submitted
06 Cig 2018
Accepted
21 Cax 2018
First published
21 Cax 2018

Metallomics, 2018,10, 794-801

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