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Issue 37, 2015
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Stabilization of Cu(i) for binding and calorimetric measurements in aqueous solution

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Abstract

Conditions have been developed for the comproportionation reaction of Cu2+ and copper metal to prepare aqueous solutions of Cu+ that are stabilized from disproportionation by MeCN and other Cu+-stabilizing ligands. These solutions were then used in ITC measurements to quantify the thermodynamics of formation of a set of Cu+ complexes (CuI(MeCN)3+, CuIMe6Trien+, CuI(BCA)23−, CuI(BCS)23−), which have stabilities ranging over 15 orders of magnitude, for their use in binding and calorimetric measurements of Cu+ interaction with proteins and other biological macromolecules. These complexes were then used to determine the stability and thermodynamics of formation of a 1 : 1 complex of Cu+ with the biologically important tri-peptide glutathione, GSH. These results identify Me6Trien as an attractive Cu+-stabilizing ligand for calorimetric experiments, and suggest that caution should be used with MeCN to stabilize Cu+ due to its potential for participating in unquantifiable ternary interactions.

Graphical abstract: Stabilization of Cu(i) for binding and calorimetric measurements in aqueous solution

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Supplementary files

Article information


Submitted
15 Jul 2015
Accepted
21 Aug 2015
First published
24 Aug 2015

Dalton Trans., 2015,44, 16494-16505
Article type
Paper
Author version available

Stabilization of Cu(I) for binding and calorimetric measurements in aqueous solution

D. K. Johnson, M. J. Stevenson, Z. A. Almadidy, S. E. Jenkins, Dean. E. Wilcox and N. E. Grossoehme, Dalton Trans., 2015, 44, 16494
DOI: 10.1039/C5DT02689J

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