Issue 36, 2016

Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

Abstract

A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.

Graphical abstract: Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

Supplementary files

Article information

Article type
Communication
Submitted
26 Feb 2016
Accepted
05 Apr 2016
First published
07 Apr 2016
This article is Open Access
Creative Commons BY license

Chem. Commun., 2016,52, 6158-6161

Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

M. J. Weissenborn, S. Notonier, S. Lang, K. B. Otte, S. Herter, N. J. Turner, S. L. Flitsch and B. Hauer, Chem. Commun., 2016, 52, 6158 DOI: 10.1039/C6CC01749E

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