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Issue 36, 2016
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Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

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Abstract

A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.

Graphical abstract: Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

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Supplementary files

Article information


Submitted
26 Feb 2016
Accepted
05 Apr 2016
First published
07 Apr 2016

This article is Open Access

Chem. Commun., 2016,52, 6158-6161
Article type
Communication

Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

M. J. Weissenborn, S. Notonier, S. Lang, K. B. Otte, S. Herter, N. J. Turner, S. L. Flitsch and B. Hauer, Chem. Commun., 2016, 52, 6158
DOI: 10.1039/C6CC01749E

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