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Issue 19, 2015
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Development of an automated on-chip bead-based ELISA platform

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Abstract

We present a lab-on-a-chip and associated instrument for heterogeneous enzyme-linked immunosorbent assay (ELISA)-based detection of proteins from liquid samples. The system performs all necessary ELISA steps (starting from antigen incubation) in a quarter of the time required for corresponding plate-based protocols. We have previously described the instrument, which automates fluidic control via remote valve switching and detects fluorescence from reacted substrate, for use in a molecular diagnostics application. The ELISA chip reported here utilizes a high surface area bead bed to enhance capture efficiency and increase the dynamic range of the assay as compared to a standard plate-based ELISA. Its functionality is demonstrated using human IL-10 as a model antigen, but theoretically any sandwich ELISA could be ported onto this “open source platform.” We show that our automated on-chip assays have greater sensitivities than the corresponding standard manual plate-based ELISAs, and that single samples can be assayed in a fraction of the time.

Graphical abstract: Development of an automated on-chip bead-based ELISA platform

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Publication details

The article was received on 29 Jan 2015, accepted on 12 Aug 2015 and first published on 13 Aug 2015


Article type: Technical Note
DOI: 10.1039/C5AY00264H
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Citation: Anal. Methods, 2015,7, 8472-8477
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    Development of an automated on-chip bead-based ELISA platform

    J. Campbell, N. R. Pollock, A. Sharon and A. F. Sauer-Budge, Anal. Methods, 2015, 7, 8472
    DOI: 10.1039/C5AY00264H

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