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Issue 7, 2014
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A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer readout

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Abstract

A portable and quantitative enzyme immunoassay with a glucometer readout was developed for the sensitive monitoring of neuron-specific enolase (NSE, as a model analyte) in a high-binding polystyrene 96-well microtiter plate (MTP), conjugated with monoclonal mouse anti-human NSE antibody (mAb1). Gold nanoparticles heavily functionalized with glucoamylase and polyclonal rabbit anti-human NSE antibody (pAb2) were utilized as the trace tag. A sandwich-type immunoassay format was adopted for the quantitative detection of NSE in the mAb1-functionalized MTP. Accompanying the gold nanoparticles, the carried glucoamylase could hydrolyze amylopectin in glucose. The produced glucose could be quantitatively monitored using a portable personal glucose meter (PGM). Under optimal conditions, the PGM-based immunoassay exhibited good analytical properties for the determination of the target NSE, and allowed the detection of NSE at concentrations as low as 0.05 ng mL−1. The intra- and inter-assay coefficients of variation (CVs) were below 10% and 11%, respectively. The methodology was also evaluated by assaying 15 clinical serum samples, and showed good accordance between results obtained by the developed immunoassay and the referenced values.

Graphical abstract: A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer readout

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Article information


Submitted
21 Nov 2013
Accepted
19 Jan 2014
First published
20 Jan 2014

Anal. Methods, 2014,6, 2233-2238
Article type
Paper
Author version available

A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer readout

X. Fu, X. Feng, K. Xu and R. Huang, Anal. Methods, 2014, 6, 2233
DOI: 10.1039/C3AY42075B

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