Matrix solid-phase dispersion: a simple and fast technique for the determination of phenolic compounds in olive oil by liquid chromatography

Abstract

A methodology was developed using matrix solid-phase dispersion, together with liquid chromatography with multiple wavelength detectors for the determination of 20 phenolic compounds in olive oil samples. Under optimized conditions, the analytes were extracted using 0.5 g of olive oil, 1.0 g of Florisil as a sorbent and 1 mL methanol–water (80 : 20 acidified with formic acid 0.5% (v/v)) as an eluting solvent. The proposed methodology provided detection and quantification limits of individual compounds in the ranges of 0.02–0.75 and 0.08–2.50 mg kg⁻¹, respectively. The RSDs resulting from the analysis of six replicates of 0.5 g of a sample pool containing 2.5 mg kg⁻¹ phenolic compounds were ranged between 2.1% and 14.8%. Considering matrix-matched calibration as a quantification technique, the average recoveries ranged from 74.8% to 95.0% with relative standard deviations between 1.5% and 9.3%. The developed methodology was applied for the determination of phenolic compounds in nine olive oils produced in Argentina, identifying seventeen analytes at concentrations above the detectable levels.