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Issue 1, 2021
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Post-translational modifications soften vimentin intermediate filaments

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Abstract

The mechanical properties of biological cells are determined by the cytoskeleton, a composite biopolymer network consisting of microtubules, actin filaments and intermediate filaments (IFs). By differential expression of cytoskeletal proteins, modulation of the network architecture and interactions between the filaments, cell mechanics may be adapted to varying requirements on the cell. Here, we focus on the intermediate filament protein vimentin and introduce post-translational modifications as an additional, much faster mechanism for mechanical modulation. We study the impact of phosphorylation on filament mechanics by recording force-strain curves using optical traps. Partial phosphorylation softens the filaments. We show that binding of the protein 14-3-3 to phosphorylated vimentin IFs further enhances this effect and speculate that in the cell 14-3-3 may serve to preserve the softening and thereby the altered cell mechanics. We explain our observation by the additional charges introduced during phosphorylation.

Graphical abstract: Post-translational modifications soften vimentin intermediate filaments

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Supplementary files

Article information


Submitted
13 Oct 2020
Accepted
09 Dec 2020
First published
10 Dec 2020

This article is Open Access

Nanoscale, 2021,13, 380-387
Article type
Paper

Post-translational modifications soften vimentin intermediate filaments

J. Kraxner, C. Lorenz, J. Menzel, I. Parfentev, I. Silbern, M. Denz, H. Urlaub, B. Schwappach and S. Köster, Nanoscale, 2021, 13, 380
DOI: 10.1039/D0NR07322A

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    [Original citation] - Published by The Royal Society of Chemistry.

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