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Issue 24, 2020
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In cell Gd3+-based site-directed spin labeling and EPR spectroscopy of eGFP

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Abstract

Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells. In cell spin labeling using alkyne–azide click chemistry with a Gd3+-DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscopic detection of a spin labeled protein in living cells at ambient temperatures.

Graphical abstract: In cell Gd3+-based site-directed spin labeling and EPR spectroscopy of eGFP

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Supplementary files

Article information


Submitted
09 Apr 2020
Accepted
04 May 2020
First published
01 Jun 2020

This article is Open Access

Phys. Chem. Chem. Phys., 2020,22, 13358-13362
Article type
Communication

In cell Gd3+-based site-directed spin labeling and EPR spectroscopy of eGFP

S. Kucher, S. Korneev, J. P. Klare, D. Klose and H. Steinhoff, Phys. Chem. Chem. Phys., 2020, 22, 13358
DOI: 10.1039/D0CP01930E

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