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Issue 8, 2017
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A convenient method for multicolour labelling of proteins with BODIPY fluorophores via tyrosine residues

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Abstract

Fluorescence is an essential imaging modality for labelling and visualising cells and sub-cellular structures. Multicolour labelling is especially challenging due to differences in physicochemical and photophysical behaviour of structurally unrelated fluorophores in the heterogeneous environments found in sub-cellular compartments. Herein, we report the conjugation of three azide-bearing BODIPYs with similar core structures but widely different emission wavelengths (green, red and NIR) to tyrosine residues of a model globular protein (BSA) via a common linking methodology. The resulting BODIPY–BSA conjugates have demonstrated multi-wavelength fluorescence emission for biological applications. Fluorescence imaging was performed in HeLa cells through live cell confocal microscopy imaging, with good intracellular location visualisation observed.

Graphical abstract: A convenient method for multicolour labelling of proteins with BODIPY fluorophores via tyrosine residues

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Supplementary files

Article information


Submitted
09 Mar 2017
Accepted
28 May 2017
First published
31 May 2017

Photochem. Photobiol. Sci., 2017,16, 1260-1267
Article type
Paper

A convenient method for multicolour labelling of proteins with BODIPY fluorophores via tyrosine residues

Miffy. H. Y. Cheng, H. Savoie, F. Bryden and Ross. W. Boyle, Photochem. Photobiol. Sci., 2017, 16, 1260
DOI: 10.1039/C7PP00091J

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