Jump to main content
Jump to site search

Issue 16, 2017
Previous Article Next Article

3D material cytometry (3DMaC): a very high-replicate, high-throughput analytical method using microfabricated, shape-specific, cell-material niches

Author affiliations

Abstract

Studying cell behavior within 3D material niches is key to understanding cell biology in health and diseases, and developing biomaterials for regenerative medicine applications. Current approaches to studying these cell–material niches have low throughput and can only analyze a few replicates per experiment resulting in reduced measurement assurance and analytical power. Here, we report 3D material cytometry (3DMaC), a novel high-throughput method based on microfabricated, shape-specific 3D cell–material niches and imaging cytometry. 3DMaC achieves rapid and highly multiplexed analyses of very high replicate numbers (“n” of 104–106) of 3D biomaterial constructs. 3DMaC overcomes current limitations of low “n”, low-throughput, and “noisy” assays, to provide rapid and simultaneous analyses of potentially hundreds of parameters in 3D biomaterial cultures. The method is demonstrated here for a set of 85 000 events containing twelve distinct cell–biomaterial micro-niches along with robust, customized computational methods for high-throughput analytics with potentially unprecedented statistical power.

Graphical abstract: 3D material cytometry (3DMaC): a very high-replicate, high-throughput analytical method using microfabricated, shape-specific, cell-material niches

Back to tab navigation

Supplementary files

Publication details

The article was received on 25 Apr 2017, accepted on 29 Jun 2017 and first published on 20 Jul 2017


Article type: Paper
DOI: 10.1039/C7LC00451F
Lab Chip, 2017,17, 2861-2872

  •   Request permissions

    3D material cytometry (3DMaC): a very high-replicate, high-throughput analytical method using microfabricated, shape-specific, cell-material niches

    K. Parratt, J. Jeong, P. Qiu and K. Roy, Lab Chip, 2017, 17, 2861
    DOI: 10.1039/C7LC00451F

Search articles by author

Spotlight

Advertisements