Jump to main content
Jump to site search

Issue 17, 2016
Previous Article Next Article

Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

Author affiliations

Abstract

A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered.

Graphical abstract: Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

Back to tab navigation

Supplementary files

Article information


Submitted
10 Mar 2016
Accepted
01 Apr 2016
First published
04 Apr 2016

Org. Biomol. Chem., 2016,14, 4023-4026
Article type
Communication

Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

M. Liras, S. Simoncelli, A. Rivas-Aravena, O. García, J. C. Scaiano, E. I. Alarcon and A. Aspée, Org. Biomol. Chem., 2016, 14, 4023
DOI: 10.1039/C6OB00533K

Social activity

Search articles by author

Spotlight

Advertisements