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Issue 15, 2016
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Human immune cell targeting of protein nanoparticles – caveospheres

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Abstract

Nanotechnology has the power to transform vaccine and drug delivery through protection of payloads from both metabolism and off-target effects, while facilitating specific delivery of cargo to immune cells. However, evaluation of immune cell nanoparticle targeting is conventionally restricted to monocultured cell line models. We generated human caveolin-1 nanoparticles, termed caveospheres, which were efficiently functionalized with monoclonal antibodies. Using this platform, we investigated CD4+ T cell and CD20+ B cell targeting within physiological mixtures of primary human blood immune cells using flow cytometry, imaging flow cytometry and confocal microscopy. Antibody-functionalization enhanced caveosphere binding to targeted immune cells (6.6 to 43.9-fold) within mixed populations and in the presence of protein-containing fluids. Moreover, targeting caveospheres to CCR5 enabled caveosphere internalization by non-phagocytic CD4+ T cells—an important therapeutic target for HIV treatment. This efficient and flexible system of immune cell-targeted caveosphere nanoparticles holds promise for the development of advanced immunotherapeutics and vaccines.

Graphical abstract: Human immune cell targeting of protein nanoparticles – caveospheres

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Publication details

The article was received on 20 Jan 2016, accepted on 22 Mar 2016 and first published on 31 Mar 2016


Article type: Paper
DOI: 10.1039/C6NR00506C
Citation: Nanoscale, 2016,8, 8255-8265
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    Human immune cell targeting of protein nanoparticles – caveospheres

    J. J. Glass, D. Yuen, J. Rae, A. P. R. Johnston, R. G. Parton, S. J. Kent and R. De Rose, Nanoscale, 2016, 8, 8255
    DOI: 10.1039/C6NR00506C

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