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Issue 12, 2016

Raman spectroscopy as a tool for label-free lymphocyte cell line discrimination

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Abstract

Unactivated lymphocytes are morphologically identical and biochemically relatively similar, making them difficult to distinguish from one another with conventional light microscopy. Here, we use Raman spectroscopy to provide biochemical information on the composition of different lymphocyte cell lines. As could be expected, the biochemical differences measured with Raman spectroscopy between lymphocyte cell lines are small, but in combination with partial least squares discriminant analysis it is possible not only to distinguish between T- and B-cells, but also between individual T-cell and B-cell lines.

Graphical abstract: Raman spectroscopy as a tool for label-free lymphocyte cell line discrimination

Supplementary files

Article information


Submitted
25 Jan 2016
Accepted
24 Mar 2016
First published
24 Mar 2016

Analyst, 2016,141, 3756-3764
Article type
Paper

Raman spectroscopy as a tool for label-free lymphocyte cell line discrimination

A. J. Hobro, Y. Kumagai, S. Akira and N. I. Smith, Analyst, 2016, 141, 3756 DOI: 10.1039/C6AN00181E

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