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Issue 12, 2016
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Raman spectroscopy as a tool for label-free lymphocyte cell line discrimination

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Abstract

Unactivated lymphocytes are morphologically identical and biochemically relatively similar, making them difficult to distinguish from one another with conventional light microscopy. Here, we use Raman spectroscopy to provide biochemical information on the composition of different lymphocyte cell lines. As could be expected, the biochemical differences measured with Raman spectroscopy between lymphocyte cell lines are small, but in combination with partial least squares discriminant analysis it is possible not only to distinguish between T- and B-cells, but also between individual T-cell and B-cell lines.

Graphical abstract: Raman spectroscopy as a tool for label-free lymphocyte cell line discrimination

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Publication details

The article was received on 25 Jan 2016, accepted on 24 Mar 2016 and first published on 24 Mar 2016


Article type: Paper
DOI: 10.1039/C6AN00181E
Analyst, 2016,141, 3756-3764

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    Raman spectroscopy as a tool for label-free lymphocyte cell line discrimination

    A. J. Hobro, Y. Kumagai, S. Akira and N. I. Smith, Analyst, 2016, 141, 3756
    DOI: 10.1039/C6AN00181E

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