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Issue 12, 2015
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One-step purification and concentration of DNA in porous membranes for point-of-care applications

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Abstract

The emergence of rapid, user-friendly, point-of-care (POC) diagnostic systems is paving the way for better disease diagnosis and control. Lately, there has been a strong emphasis on developing molecular-based diagnostics due to their potential for greatly increased sensitivity and specificity. One of the most critical steps in developing practical diagnostic systems is the ability to perform sample preparation, especially the purification of nucleic acids (NA), at the POC. As such, we have developed a simple-to-use, inexpensive, and disposable sample preparation system for in-membrane purification and concentration of NAs. This system couples lateral flow in a porous membrane with chitosan, a linear polysaccharide that captures NAs via anion exchange chromatography. The system can also substantially concentrate the NAs. The combination of these capabilities can be used on a wide range of sample types, which are prepared for use in downstream processes, such as qPCR, without further purification.

Graphical abstract: One-step purification and concentration of DNA in porous membranes for point-of-care applications

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Supplementary files

Article information


Submitted
16 Mar 2015
Accepted
08 May 2015
First published
08 May 2015

Lab Chip, 2015,15, 2647-2659
Article type
Paper
Author version available

One-step purification and concentration of DNA in porous membranes for point-of-care applications

S. A. Byrnes, J. D. Bishop, L. Lafleur, J. R. Buser, B. Lutz and P. Yager, Lab Chip, 2015, 15, 2647
DOI: 10.1039/C5LC00317B

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