Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 12, 2015
Previous Article Next Article

One-step purification and concentration of DNA in porous membranes for point-of-care applications

Author affiliations

Abstract

The emergence of rapid, user-friendly, point-of-care (POC) diagnostic systems is paving the way for better disease diagnosis and control. Lately, there has been a strong emphasis on developing molecular-based diagnostics due to their potential for greatly increased sensitivity and specificity. One of the most critical steps in developing practical diagnostic systems is the ability to perform sample preparation, especially the purification of nucleic acids (NA), at the POC. As such, we have developed a simple-to-use, inexpensive, and disposable sample preparation system for in-membrane purification and concentration of NAs. This system couples lateral flow in a porous membrane with chitosan, a linear polysaccharide that captures NAs via anion exchange chromatography. The system can also substantially concentrate the NAs. The combination of these capabilities can be used on a wide range of sample types, which are prepared for use in downstream processes, such as qPCR, without further purification.

Graphical abstract: One-step purification and concentration of DNA in porous membranes for point-of-care applications

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 Mar 2015, accepted on 08 May 2015 and first published on 08 May 2015


Article type: Paper
DOI: 10.1039/C5LC00317B
Author version
available:
Download author version (PDF)
Citation: Lab Chip, 2015,15, 2647-2659

  •   Request permissions

    One-step purification and concentration of DNA in porous membranes for point-of-care applications

    S. A. Byrnes, J. D. Bishop, L. Lafleur, J. R. Buser, B. Lutz and P. Yager, Lab Chip, 2015, 15, 2647
    DOI: 10.1039/C5LC00317B

Search articles by author

Spotlight

Advertisements