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Issue 2, 2015
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Marker-free detection of progenitor cell differentiation by analysis of Brownian motion in micro-wells

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The kinetics of stem and progenitor cell differentiation at the single-cell level provides essential clues to the complexity of the underlying decision-making circuits. In many hematopoietic progenitor cells, differentiation is accompanied by the expression of lineage-specific markers and by a transition from a non-adherent to an adherent state. Here, using the granulocyte-macrophage progenitor (GMP) as a model, we introduce a label-free approach that allows one to follow the course of this transition in hundreds of single cells in parallel. We trap single cells in patterned arrays of micro-wells and use phase-contrast time-lapse movies to distinguish non-adherent from adherent cells by an analysis of Brownian motion. This approach allowed us to observe the kinetics of induced differentiation of primary bone-marrow-derived GMPs into macrophages. The time lapse started 2 hours after addition of the cytokine M-CSF, and nearly 80% of the population had accomplished the transition within the first 20 h. The analysis of Brownian motion proved to be a sensitive and robust tool for monitoring the transition, and thus provides a high-throughput method for the study of cell differentiation at the single-cell level.

Graphical abstract: Marker-free detection of progenitor cell differentiation by analysis of Brownian motion in micro-wells

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Supplementary files

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The article was received on 07 Jul 2014, accepted on 27 Nov 2014 and first published on 09 Dec 2014

Article type: Paper
DOI: 10.1039/C4IB00158C
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Citation: Integr. Biol., 2015,7, 178-183

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