Jump to main content
Jump to site search

Issue 2, 2015
Previous Article Next Article

Marker-free detection of progenitor cell differentiation by analysis of Brownian motion in micro-wells

Author affiliations

Abstract

The kinetics of stem and progenitor cell differentiation at the single-cell level provides essential clues to the complexity of the underlying decision-making circuits. In many hematopoietic progenitor cells, differentiation is accompanied by the expression of lineage-specific markers and by a transition from a non-adherent to an adherent state. Here, using the granulocyte-macrophage progenitor (GMP) as a model, we introduce a label-free approach that allows one to follow the course of this transition in hundreds of single cells in parallel. We trap single cells in patterned arrays of micro-wells and use phase-contrast time-lapse movies to distinguish non-adherent from adherent cells by an analysis of Brownian motion. This approach allowed us to observe the kinetics of induced differentiation of primary bone-marrow-derived GMPs into macrophages. The time lapse started 2 hours after addition of the cytokine M-CSF, and nearly 80% of the population had accomplished the transition within the first 20 h. The analysis of Brownian motion proved to be a sensitive and robust tool for monitoring the transition, and thus provides a high-throughput method for the study of cell differentiation at the single-cell level.

Graphical abstract: Marker-free detection of progenitor cell differentiation by analysis of Brownian motion in micro-wells

Back to tab navigation

Supplementary files

Publication details

The article was received on 07 Jul 2014, accepted on 27 Nov 2014 and first published on 09 Dec 2014


Article type: Paper
DOI: 10.1039/C4IB00158C
Author version
available:
Download author version (PDF)
Citation: Integr. Biol., 2015,7, 178-183

Search articles by author

Spotlight

Advertisements