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Issue 73, 2014
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Ultra-stable glass microcraters for on-chip patch clamping

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Abstract

We report on a single-step fabrication procedure of borosilicate glass micropores surrounded by a smooth microcrater. By inserting a thin air-gap between a borosilicate glass substrate and a reflective layer, we achieve dual-sided laser ablation of the device. The resultant crater provides a smoother, curved surface onto which cells settle during planar patch clamping. Gigaohm seals, which are more easily achievable on these devices as compared to conventional micropores, are achieved by patch clamping human embryonic kidney (HEK 293) cells. Further, the microcraters show enhanced mechanical stability of the planar patch clamped cells during perfusion. We integrate polydimethylsiloxane microfluidic devices with the microcraters and use passive pumping to perfuse the cells. We find that passive pumping increases the pressure within the device by 1.85 Pa. However, due to the enhanced stability of the microcrater, fluidic shearing reduces the seal resistance by only 6.8 MΩ on average, which is less than one percent of the gigaohm seal resistance.

Graphical abstract: Ultra-stable glass microcraters for on-chip patch clamping

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Supplementary files

Article information


Submitted
26 May 2014
Accepted
13 Aug 2014
First published
13 Aug 2014

RSC Adv., 2014,4, 39073-39076
Article type
Communication
Author version available

Ultra-stable glass microcraters for on-chip patch clamping

E. Stava, H. C. Shin, M. Yu, A. Bhat, P. Resto, A. Seshadri, J. C. Williams and R. H. Blick, RSC Adv., 2014, 4, 39073
DOI: 10.1039/C4RA04978K

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