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Issue 81, 2014
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Monitoring ATP hydrolysis and ATPase inhibitor screening using 1H NMR

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Abstract

We present a versatile method to characterize ATPase and kinase activities and discover new inhibitors of these proteins. The proton NMR-based assay directly monitors ATP turnover and is easy to implement, requires no additional reagents and can potentially be applied to GTP. We validated the method's accuracy, applied it to the monitoring of ATP turnover by actin and to the screening of ATPase inhibitors, and showed that it is also applicable for the monitoring of GTP hydrolysis.

Graphical abstract: Monitoring ATP hydrolysis and ATPase inhibitor screening using 1H NMR

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Article information


Submitted
09 Jun 2014
Accepted
22 Aug 2014
First published
29 Aug 2014

Chem. Commun., 2014,50, 12037-12039
Article type
Communication

Monitoring ATP hydrolysis and ATPase inhibitor screening using 1H NMR

B. Guo, P. S. Gurel, R. Shu, H. N. Higgs, M. Pellegrini and D. F. Mierke, Chem. Commun., 2014, 50, 12037
DOI: 10.1039/C4CC04399E

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