Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 20, 2013
Previous Article Next Article

The global identification of tRNA isoacceptors by targeted tandem mass spectrometry

Author affiliations

Abstract

Transfer ribonucleic acids (tRNA) are a biologically significant class of non-coding ribonucleic acids (ncRNAs) that pose unique analytical challenges for complete characterization. Here we present a robust and simple method for the consistent and accurate identification of individual tRNAs from a pool of total tRNA obtained from cell lysate. Through this method individual isoacceptor tRNAs are identified by the detection of unique oligonucleotide sequences which arise from a single enzymatic digestion. These unique sequences can be detected by monitoring specific transitions from precursor to product ions. Thus, for any pool of known tRNA sequences including posttranscriptional modifications, targeted tandem mass spectrometry can be used for monitoring these specific transitions. The proposed method was developed and validated using a set of known tRNAs from Escherichia coli. This approach was found to identify 41 ± 2 of the predicted 47 isoaccepting tRNAs in E. coli from targeted tandem mass spectrometry using only 24 precursor m/z values. This method should be easily adapted to other bacterial systems for both genomic and posttranscriptional analysis of tRNAs, and is likely suitable for future clinical applications.

Graphical abstract: The global identification of tRNA isoacceptors by targeted tandem mass spectrometry

Back to tab navigation

Supplementary files

Article information


Submitted
22 Jun 2013
Accepted
01 Aug 2013
First published
05 Aug 2013

Analyst, 2013,138, 6063-6072
Article type
Paper

The global identification of tRNA isoacceptors by targeted tandem mass spectrometry

C. Wetzel and P. A. Limbach, Analyst, 2013, 138, 6063
DOI: 10.1039/C3AN01224G

Social activity

Search articles by author

Spotlight

Advertisements