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Issue 4, 2012
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Bioinformatic and experimental fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells

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Abstract

Determining interacting cellular partners of drugs by chemical proteomic techniques is complex and tedious. Most approaches rely on activity-based probe profiling and compound-centric chemical proteomics. The anti-malarial artemisinin also exerts profound anti-cancer activity, but the mechanisms of action are incompletely understood. In the present investigation, we present a novel approach to identify artemisinin-interacting target proteins. Our approach overcomes usual problems in traditional fishing procedures, because the drug was attached to a surface without further chemical modification. The proteins identified effect among others, cell cycle arrest, apoptosis, inhibition of angiogenesis, disruption of cell migration, and modulation of nuclear receptor responsiveness. Furthermore, a bioinformatic approach confirmed experimentally identified proteins and suggested a large number of other interacting proteins. Theoretically predicted interaction partners may serve as a starting point to complete the whole set of proteins binding artemisinin.

Graphical abstract: Bioinformatic and experimental fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells

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Article information


Submitted
23 Oct 2011
Accepted
10 Jan 2012
First published
06 Feb 2012

Mol. BioSyst., 2012,8, 1311-1318
Article type
Paper

Bioinformatic and experimental fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells

T. Eichhorn, S. Schloissnig, B. Hahn, A. Wendler, R. Mertens, W. D. Lehmann, R. L. Krauth-Siegel and T. Efferth, Mol. BioSyst., 2012, 8, 1311
DOI: 10.1039/C2MB05437J

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