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Issue 21, 2012
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Droplet electroporation in microfluidics for efficient cell transformation with or without cell wall removal

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Abstract

An efficient cell transformation method is presented that utilizes droplet electroporation on a microfluidic chip. Two types of green microalgae, a wall-less mutant and a wild type of Chlamydomonas reinhardtii, are used as model cells. The PDMS-glass electroporation chip is simply composed of a flow-focusing microstructure for generating cell-encapsulating droplets and a serpentine channel for better mixing of the content in the droplet, and five pairs of parallel microelectrodes on the glass slide, without involving any expensive electrical equipment. The transformation efficiency via the microfluidic electroporation is shown to be more than three orders of magnitude higher for the wall-less mutant, and more than two orders of magnitude higher for the wild type, which has its cell wall intact, than bulk phase electroporation under identical conditions. Furthermore, the microfluidic transformation is remarkably efficient even at a low DNA/cell ratio, facilitating ways of controlling the transgenic copy number, which is important for the stability of the transgene expression.

Graphical abstract: Droplet electroporation in microfluidics for efficient cell transformation with or without cell wall removal

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Publication details

The article was received on 15 Apr 2012, accepted on 20 Aug 2012 and first published on 21 Aug 2012


Article type: Paper
DOI: 10.1039/C2LC40360A
Citation: Lab Chip, 2012,12, 4483-4488
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    Droplet electroporation in microfluidics for efficient cell transformation with or without cell wall removal

    B. Qu, Y. Eu, W. Jeong and D. Kim, Lab Chip, 2012, 12, 4483
    DOI: 10.1039/C2LC40360A

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