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Issue 18, 2011
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Optical acetylcholine sensor based on free base porphyrin as a chromoionophore

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Abstract

In this work, the possibility of application of free base porphyrin as a lipophilic pH chromoionophore for the preparation of optical cation-selective sensors was investigated. The properties of polymeric membranes, containing porphyrins of different structures, namely tetraphenylporphyrin (TPP) and octaethylporphyrin (OEP), were compared. Changes in equilibrium between protonated and deprotonated form of porphyrin, resulting from variations in ACh concentration, were evaluated. The influence of various factors (kind and quantity of anionic additive and porphyrin in the membrane phase, pH of sample solution) on initial equilibrium was studied. The best membrane composition was chosen as: TPP 3 wt.%, KTFPB 175 mol.% relative to ionophore, PVC:o-NPOE (1 : 4) and measuring buffer solution: 0.05 M MES, pH 4.5. Selectivity, response stability, reversibility and repeatability tests were carried out for chosen sensor. Developed sensor allowed for the determination of a model analyte, acetylcholine, at the concentration range of 10−5 to 10−2 M, both in stationary and flow-injection system. Sensor response was reversible and repeatable in the mentioned concentration range.

Graphical abstract: Optical acetylcholine sensor based on free base porphyrin as a chromoionophore

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Publication details

The article was received on 02 Dec 2010, accepted on 06 Jun 2011 and first published on 21 Jul 2011


Article type: Paper
DOI: 10.1039/C0AN00965B
Analyst, 2011,136, 3770-3776

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    Optical acetylcholine sensor based on free base porphyrin as a chromoionophore

    M. Mroczkiewicz, M. Pietrzak, Ł. Górski and E. Malinowska, Analyst, 2011, 136, 3770
    DOI: 10.1039/C0AN00965B

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