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Issue 10, 2010
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Magnetic bead-based fluorescence immunoassay for aflatoxin B1 in food using biofunctionalized rhodamine B-doped silicananoparticles

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Abstract

A simple and sensitive fluorescence immunoassay for the detection of aflatoxin B1 (AFB1, as a model compound) in food was developed using AFB1–bovine serum albumin conjugate (AFB1–BSA)-functionalized magnetic beads as immunosensing probes. The recognition elements were prepared by doping of rhodamine B (RB) fluorophore into silica nanoparticles followed by immobilization of monoclonal anti-AFB1 antibodies on the silica shell. Based on a competitive-type immunoassay format, the assay was performed both in low-binding polypropylene 96-well microtiter plates (MTPs) and in an automated sequential injection (SI) format. Similar detection limit (LOD) of 0.2 ng mL−1vs. 0.1 ng mL−1 but narrower dynamic working linear range of 0.5–7 ng mL−1vs. 0.5–30 ng mL−1 was obtained toward AFB1 standards with the flow setup compared to the MTP format. Intra-batch assay precision was substantially improved (≤5.3% vs. ≤8.7%) by resorting to the SI manifold. The proposed method features unbiased identification of negative (blank) and positive samples. No significant differences at the 95% confidence level were encountered in the analysis of naturally contaminated peanut samples between the proposed immunoassay and liquid chromatography for determination of AFB1.

Graphical abstract: Magnetic bead-based fluorescence immunoassay for aflatoxin B1 in food using biofunctionalized rhodamine B-doped silica nanoparticles

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Article information


Submitted
09 Apr 2010
Accepted
21 Jul 2010
First published
27 Aug 2010

Analyst, 2010,135, 2661-2667
Article type
Paper

Magnetic bead-based fluorescence immunoassay for aflatoxin B1 in food using biofunctionalized rhodamine B-doped silica nanoparticles

D. Tang, Y. Yu, R. Niessner, M. Miró and D. Knopp, Analyst, 2010, 135, 2661
DOI: 10.1039/C0AN00221F

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