Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 21st October 2020 from 07:00 AM to 07:00 PM (BST).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 3, 2004
Previous Article Next Article

Power-free poly(dimethylsiloxane) microfluidic devices for gold nanoparticle-based DNA analysis

Author affiliations

Abstract

An extremely simple, power-free pumping method for poly(dimethylsiloxane) (PDMS) microfluidic devices is presented. By exploiting the high gas solubility of PDMS, the energy for the pumping is pre-stored in the degassed bulk PDMS, therefore no additional structures other than channels and reservoirs are required. In a Y-shaped microchannel with cross section of 100 µm width × 25 µm height, this method has provided flow rate of 0.5–2 nL s−1, corresponding to linear velocity of 0.2–0.8 mm s−1, with good reproducibility. As an application of the power-free pumping, gold nanoparticle-based DNA analysis, which does not rely on the cross-linking mechanism between nanoparticles, has been implemented in a microchannel with three inlets. Target 15mer DNA has been easily and unambiguously discriminated from its single-base substituted mutant. Instead of colorimetric detection in a conventional microtube, an alternative detection technique suitable for microdevices has been discovered—observation of deposition on the PDMS surfaces. The channel layout enabled two simultaneous DNA analyses at the two interfaces between the three laminar streams.

Back to tab navigation

Supplementary files

Article information


Submitted
15 Mar 2004
Accepted
15 Apr 2004
First published
12 May 2004

Lab Chip, 2004,4, 181-185
Article type
Paper

Power-free poly(dimethylsiloxane) microfluidic devices for gold nanoparticle-based DNA analysis

K. Hosokawa, K. Sato, N. Ichikawa and M. Maeda, Lab Chip, 2004, 4, 181
DOI: 10.1039/B403930K

Social activity

Search articles by author

Spotlight

Advertisements