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Issue 3, 2004
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Power-free poly(dimethylsiloxane) microfluidic devices for gold nanoparticle-based DNA analysis

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An extremely simple, power-free pumping method for poly(dimethylsiloxane) (PDMS) microfluidic devices is presented. By exploiting the high gas solubility of PDMS, the energy for the pumping is pre-stored in the degassed bulk PDMS, therefore no additional structures other than channels and reservoirs are required. In a Y-shaped microchannel with cross section of 100 µm width × 25 µm height, this method has provided flow rate of 0.5–2 nL s−1, corresponding to linear velocity of 0.2–0.8 mm s−1, with good reproducibility. As an application of the power-free pumping, gold nanoparticle-based DNA analysis, which does not rely on the cross-linking mechanism between nanoparticles, has been implemented in a microchannel with three inlets. Target 15mer DNA has been easily and unambiguously discriminated from its single-base substituted mutant. Instead of colorimetric detection in a conventional microtube, an alternative detection technique suitable for microdevices has been discovered—observation of deposition on the PDMS surfaces. The channel layout enabled two simultaneous DNA analyses at the two interfaces between the three laminar streams.

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Supplementary files

Article information

15 Mar 2004
15 Apr 2004
First published
12 May 2004

Lab Chip, 2004,4, 181-185
Article type

Power-free poly(dimethylsiloxane) microfluidic devices for gold nanoparticle-based DNA analysis

K. Hosokawa, K. Sato, N. Ichikawa and M. Maeda, Lab Chip, 2004, 4, 181
DOI: 10.1039/B403930K

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