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Issue 37, 2015
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Luciferase gene-loaded CS-Qdots as self-illuminating probes for specific hepatoma imaging

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Abstract

Chitosan encapsulated quantum dots (CS-Qdots) exhibit fascinating optical properties and can efficiently deliver genes into cells in a visualized process. By using CS-Qdots as gene carriers, specific hepatocellular carcinoma (HCC) expressed firefly luciferase genes (p[HRE]AFP-luc) were transfected into HCC cells for hepatoma bioluminescence imaging. The results obtained in this study show that nanocarrier CS-Qdots can be excited by the luciferase coded in the genes delivered into the cells. The maximum emission wavelength of the bioluminescence red-shifted from 560 nm to 630 nm. The excitation of CS-Qdots by bioluminescence occurs at the macroscopic scale and is independent of covalent bond. The luciferase gene-loaded CS-Qdots can act as wavelength-tunable self-illuminating probes thus holding potential for improved tumor optical molecular imaging.

Graphical abstract: Luciferase gene-loaded CS-Qdots as self-illuminating probes for specific hepatoma imaging

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Article information


Submitted
26 Jan 2015
Accepted
16 Mar 2015
First published
17 Mar 2015

RSC Adv., 2015,5, 29048-29057
Article type
Paper

Luciferase gene-loaded CS-Qdots as self-illuminating probes for specific hepatoma imaging

C. Yuan, L. Wang, Y. An, G. Wu and D. Zhang, RSC Adv., 2015, 5, 29048
DOI: 10.1039/C5RA01508A

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