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Issue 38, 2013
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A proteorhodopsin-based biohybrid light-powering pH sensor

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Abstract

The biohybrid sensor is an emerging technique for multi-functional detection that utilizes the instinctive responses or interactions of biomolecules. We develop a biohybrid pH sensor by taking advantage of the pH-dependent photoelectric characteristics of proteorhodopsin (pR). The transient absorption kinetics study indicates that the photoelectric behavior of pR is attributed to the varying lifetime of the M intermediate at different environmental pH values. This pR-based biohybrid light-powering sensor with microfluidic design can achieve real-time pH detection with quick response and high sensitivity. The results of this work would shed light on pR and its potential applications.

Graphical abstract: A proteorhodopsin-based biohybrid light-powering pH sensor

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Supplementary files

Article information


Submitted
20 Jul 2013
Accepted
02 Aug 2013
First published
05 Aug 2013

Phys. Chem. Chem. Phys., 2013,15, 15821-15824
Article type
Communication

A proteorhodopsin-based biohybrid light-powering pH sensor

S. Rao, Z. Guo, D. Liang, D. Chen, Y. Wei and Y. Xiang, Phys. Chem. Chem. Phys., 2013, 15, 15821
DOI: 10.1039/C3CP52894D

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