Polyoxometalates-doped Au nanoparticles and reduced graphene oxide: A new material for the detection of uric acid in urine

Zhenyuan Bai, Chunlei Zhou, Hengbin Xu, Guangning Wang, Haijun Pang, Huiyuan Ma
Sens. Actuators, B, 2017, 243, 361 - 371
Analyte Matrix Technique

uric acid


electrochemical analysis


Subject category(s): General analytical chemistry; Sensors; Clinical and biochemical analysis; Other biochemical substances

Uric acid (UA), as vital biological substance, is desirable to be accurate detected in clinical monitoring and diagnosis. Sensitive electrochemical biosensor for detection of UA was constructed with cationic poly(diallyldimethylammonium chloride) functionalized reduced graphene oxide (PDDA-rGO) and anionic Au nanoparticles mixed with polyoxometalates clusters K8P2W16V2O62 (P2W16V2-Au). The effective combination of P2W16V2-Au and PDDA-rGO brings many advantages in electrochemical detection, involving the fast transmission of electric charges, the unimpeded pathways for diffusion and more sensing sites. Under optimized conditions, the proposed sensor showed acceptable analytical performances for UA in terms of good linearity (over the concentration range from 2.5 × 10−7 to 1.5 × 10−4 M) and lower detection limit 8.0 × 10−8 M (S/N = 3). Furthermore, the superior cycling stability, reproducibility, and selectivity make the electrochemical sensor suitable for detecting the UA in urine assay.

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