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Issue 31, 2016
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Protein separations via thermally responsive ionic block copolymer brush layers

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Abstract

Temperature-responsive (thermoresponsive) protein adsorption on a block copolymer brush layer is demonstrated. Specifically, silica beads were modified with poly(3-acrylamidopropyl trimethylammonium chloride)-b-poly(N-isopropylacrylamide) (PAPTAC-b-PIPAAm) via multi-step atom-transfer radical polymerization. The beads were characterized with X-ray photoelectron spectroscopy, carbon/hydrogen/nitrogen elemental analysis, and gel-permeation chromatography. When they were used as chromatographic packing materials, an analysis of adenosine nucleotides indicated that the ionic properties of the copolymer brush layer could be modulated by the length of the PAPTAC segment. In addition, the elution of milk serum indicated that the proteins α-lactalbumin and β-lactoglobulin adsorbed on the copolymer brush layer at high temperature; they desorbed when the temperature was reduced. Thus, separation of milk serum proteins can be performed by simply changing the column temperature. In general, the block copolymer brush-modified beads could be used as thermally responsive chromatographic matrices for protein separations.

Graphical abstract: Protein separations via thermally responsive ionic block copolymer brush layers

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Publication details

The article was received on 13 jan. 2016, accepted on 29 feb. 2016 and first published on 02 mar. 2016


Article type: Paper
DOI: 10.1039/C6RA01061J
Citation: RSC Adv., 2016,6, 26254-26263
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    Protein separations via thermally responsive ionic block copolymer brush layers

    K. Nagase, J. Kobayashi, A. Kikuchi, Y. Akiyama, H. Kanazawa and T. Okano, RSC Adv., 2016, 6, 26254
    DOI: 10.1039/C6RA01061J

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