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Issue 11, 2015
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Lab on a single microbead: an ultrasensitive detection strategy enabling microRNA analysis at the single-molecule level

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Abstract

Detection of a single nucleic acid molecule is of great significance for both fundamental biochemistry studies and clinical diagnostics. By using microRNA (miRNA) as a model target, herein, we have developed a single-microbead-based sensing (SMBS) platform, which simply enables the detection of miRNA at the single-molecule level. In this strategy, an isothermal exponential amplification reaction (EXPAR) is rationally designed towards specific miRNAs and all products of the EXPAR are integrated onto a single microbead for signal amplification and fluorescence enrichment. This pushes the detection of miRNAs down to 1 aM in a 5 μL sample, corresponding to 3 copies of the miRNA molecule. This new strategy also affords high selectivity and it is capable of distinguishing among homologous miRNA family members even with a single-base difference. Due to its ultrahigh sensitivity and selectivity, the proposed SMBS platform has been successfully applied to the detection of miRNA extracted from a single cell.

Graphical abstract: Lab on a single microbead: an ultrasensitive detection strategy enabling microRNA analysis at the single-molecule level

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Publication details

The article was received on 21 júl. 2015, accepted on 13 ágú. 2015 and first published on 20 ágú. 2015


Article type: Edge Article
DOI: 10.1039/C5SC02641E
Citation: Chem. Sci., 2015,6, 6213-6218
  • Open access: Creative Commons BY license
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    Lab on a single microbead: an ultrasensitive detection strategy enabling microRNA analysis at the single-molecule level

    X. Zhang, C. Liu, L. Sun, X. Duan and Z. Li, Chem. Sci., 2015, 6, 6213
    DOI: 10.1039/C5SC02641E

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