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Issue 11, 2015
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Cell paintballing using optically targeted coacervate microdroplets

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Abstract

We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished via active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet–membrane fusion. The facile preparation, high sequestration efficiency and inherent membrane affinity of the microdroplets make this novel “cell paintballing” technology a highly advantageous option for spatially-directed cell functionalization, with potential applications in single cell stimulation, transfection and differentiation.

Graphical abstract: Cell paintballing using optically targeted coacervate microdroplets

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Publication details

The article was received on 23 jún. 2015, accepted on 20 júl. 2015 and first published on 20 júl. 2015


Article type: Edge Article
DOI: 10.1039/C5SC02266E
Citation: Chem. Sci., 2015,6, 6106-6111
  • Open access: Creative Commons BY license
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    Cell paintballing using optically targeted coacervate microdroplets

    J. P. K. Armstrong, S. N. Olof, M. D. Jakimowicz, A. P. Hollander, S. Mann, S. A. Davis, M. J. Miles, A. J. Patil and A. W. Perriman, Chem. Sci., 2015, 6, 6106
    DOI: 10.1039/C5SC02266E

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