Jump to main content
Jump to site search

Issue 11, 2015
Previous Article Next Article

Photoresponsive immunomagnetic nanocarrier for capture and release of rare circulating tumor cells

Author affiliations

Abstract

Isolation, release and culture of rare circulating tumor cells (CTCs) may, if implemented, promote the progress of individualized anti-tumor therapies. To realize the release of CTCs without disruption of their viability for further culture and analysis, we designed an effective photocontrolled CTC capture/release system by combination of photochemistry and immunomagnetic separation. 7-Aminocoumarin was synthesized as the phototrigger to bridge the connection between the anti-EpCAM antibody and the magnetic beads. The coumarin moieties produced cleavage of a C–O bond under both ultraviolet (UV) and near-infrared (NIR) light illumination, breaking the bridge and releasing CTCs from the immunomagnetic beads. Compared with conventional immunomagnetic separation systems, the negative influence of absorbed immunomagnetic beads on further CTCs culture and analysis was effectively eliminated. The system can specifically recognize 102 MCF-7 cells in 1 mL of human whole blood samples with 90% efficiency and 85% purity. Under the irradiation of UV and NIR light, 73 ± 4% and 52 ± 6% of captured cells were released with a viability of 90% and 97%, respectively. Furthermore, this technique has been used to detect CTCs from whole blood of cancer patients with high purity. This study demonstrates that the photochemical-based immunomagnetic separation method for isolating, releasing and culturing CTCs from clinic patients may provide new opportunities for cancer diagnosis and personalized therapy.

Graphical abstract: Photoresponsive immunomagnetic nanocarrier for capture and release of rare circulating tumor cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 apr. 2015, accepted on 29 júl. 2015 and first published on 30 júl. 2015


Article type: Edge Article
DOI: 10.1039/C5SC01380A
Citation: Chem. Sci., 2015,6, 6432-6438
  • Open access: Creative Commons BY license
  •   Request permissions

    Photoresponsive immunomagnetic nanocarrier for capture and release of rare circulating tumor cells

    S. Lv, J. Wang, M. Xie, N. Lu, Z. Li, X. Yan, S. Cai, P. Zhang, W. Dong and W. Huang, Chem. Sci., 2015, 6, 6432
    DOI: 10.1039/C5SC01380A

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements