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Issue 7, 2015
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pH-responsive and switchable triplex-based DNA hydrogels

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Abstract

New methods for the preparation of reversible pH-responsive DNA hydrogels based on Hoogsteen triplex structures are described. One system consists of a hydrogel composed of duplex DNA units that bridge acrylamide chains at pH = 7.4 and undergoes dissolution at pH = 5.0 through the reconfiguration of one of the duplex bridging units into a protonated CG·C+ triplex structure. The second system consists of a hydrogel consisting of acrylamide chains crosslinked in the presence of an auxiliary strand by Hoogsteen TA·T triplex interaction at pH = 7.0. The hydrogel transforms into a liquid phase at pH = 10.0 due to the separation of the triplex bridging units. The two hydrogel systems undergo reversible and cyclic hydrogel/solution transitions by subjecting the systems to appropriate pH values. The anti-cancer drug, coralyne, binds specifically to the TA·T triplex-crosslinked hydrogel thereby increasing its stiffness. The pH-controlled release of the coralyne from the hydrogel is demonstrated.

Graphical abstract: pH-responsive and switchable triplex-based DNA hydrogels

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Publication details

The article was received on 16 feb. 2015, accepted on 12 maí 2015 and first published on 13 maí 2015


Article type: Edge Article
DOI: 10.1039/C5SC00594A
Citation: Chem. Sci., 2015,6, 4190-4195
  • Open access: Creative Commons BY-NC license
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    pH-responsive and switchable triplex-based DNA hydrogels

    J. Ren, Y. Hu, C. Lu, W. Guo, M. A. Aleman-Garcia, F. Ricci and I. Willner, Chem. Sci., 2015, 6, 4190
    DOI: 10.1039/C5SC00594A

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