Jump to main content
Jump to site search

Issue 5, 2015
Previous Article Next Article

Ratiometric detection of pH fluctuation in mitochondria with a new fluorescein/cyanine hybrid sensor

Author affiliations

Abstract

The homeostasis of mitochondrial pH (pHm) is crucial in cell physiology. Developing small-molecular fluorescent sensors for the ratiometric detection of pHm fluctuation is highly demanded yet challenging. A ratiometric pH sensor, Mito-pH, was constructed by integrating a pH-sensitive FITC fluorophore with a pH-insensitive hemicyanine group. The hemicyanine group also acts as the mitochondria targeting group due to its lipophilic cationic nature. Besides its ability to target mitochondria, this sensor provides two ratiometric pH sensing modes, the dual excitation/dual emission mode (Dex/Dem) and dual excitation (Dex) mode, and its linear and reversible ratiometric response range from pH 6.15 to 8.38 makes this sensor suitable for the practical tracking of pHm fluctuation in live cells. With this sensor, stimulated pHm fluctuation has been successfully tracked in a ratiometric manner via both fluorescence imaging and flow cytometry.

Graphical abstract: Ratiometric detection of pH fluctuation in mitochondria with a new fluorescein/cyanine hybrid sensor

Back to tab navigation

Supplementary files

Publication details

The article was received on 28 des. 2014, accepted on 16 mar. 2015 and first published on 16 mar. 2015


Article type: Edge Article
DOI: 10.1039/C4SC04021J
Citation: Chem. Sci., 2015,6, 3187-3194
  • Open access: Creative Commons BY license
  •   Request permissions

    Ratiometric detection of pH fluctuation in mitochondria with a new fluorescein/cyanine hybrid sensor

    Y. Chen, C. Zhu, J. Cen, Y. Bai, W. He and Z. Guo, Chem. Sci., 2015, 6, 3187
    DOI: 10.1039/C4SC04021J

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements