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Issue 4, 2015
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Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe

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Abstract

A series of luminescent Ir(III) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(III) complex 9, [Ir(phq)2(phen)]PF6 (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity.

Graphical abstract: Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe

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Publication details

The article was received on 29 okt. 2014, accepted on 16 nóv. 2014 and first published on 25 nóv. 2014


Article type: Edge Article
DOI: 10.1039/C4SC03319A
Citation: Chem. Sci., 2015,6, 2166-2171
  • Open access: Creative Commons BY license
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    Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe

    K. Leung, H. He, B. He, H. Zhong, S. Lin, Y. Wang, D. Ma and C. Leung, Chem. Sci., 2015, 6, 2166
    DOI: 10.1039/C4SC03319A

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