A tool for the selective sequestration of ATP and PPi to aid in-solution phosphopeptide detection assays

Abstract

The presence of small phospho-anions, such as PP_i and ATP in protein samples often complicates the robust detection of phosphoproteins by metal-based chemosensors and receptors. We herein report the development of a bis(Zn²⁺-cyclen)-triethylbenzene scaffold which can selectively sequester PP_i and ATP without affecting the detection of a di-phosphorylated peptide by a ProxyPhos chemosensor.