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Themed collection Biosynthetic Assembly Lines

14 articles
Editorial

Editorial: Biosynthetic assembly lines themed issue

From the themed collection: Biosynthetic Assembly Lines
Viewpoint

A structural model for multimodular NRPS assembly lines

This viewpoint article focuses on the structures of the dissected catalytic domains of non-ribosomal peptide synthetases and how these structural details of the NRPS machinery, integrated in a didomain and an elongation module were used to generate a model for a multimodular NRPS assembly line.

Graphical abstract: A structural model for multimodular NRPS assembly lines
From the themed collection: Biosynthetic Assembly Lines
Viewpoint

Insights into the chemical logic and enzymatic machinery of NRPS assembly lines

Appreciation that some cyclic peptide antibiotics such as gramicidin S and tyrocidine were nonribosomally synthesized has been known for 50 years.

Graphical abstract: Insights into the chemical logic and enzymatic machinery of NRPS assembly lines
From the themed collection: Biosynthetic Assembly Lines
Highlight

New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

Here we describe recent advances in DNA assembly and host engineering and highlight their applications in natural product discovery and engineering.

Graphical abstract: New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters
From the themed collection: Biosynthetic Assembly Lines
Highlight

Cyclization of polyketides and non-ribosomal peptides on and off their assembly lines

In this Highlight, we focus on some newly characterized cyclization reactions that occur on and off the PKS and NRPS assembly lines, for the purpose of elucidating template effects in various manners.

Graphical abstract: Cyclization of polyketides and non-ribosomal peptides on and off their assembly lines
From the themed collection: Biosynthetic Assembly Lines
Highlight

Stereocontrol within polyketide assembly lines

The current structural and functional data on modular polyketide synthase enzymes and their relatives has enabled the construction of physical models for how KS, KR, DH, and ER set stereocenters.

Graphical abstract: Stereocontrol within polyketide assembly lines
From the themed collection: Biosynthetic Assembly Lines
Highlight

Recent advances in the biosynthesis of unusual polyketide synthase substrates

Polyketides comprise a diverse class of natural products, with many important biological and pharmacological activities. Substrates functioning as starter units and extender units during their assembly significantly contribute to the chemical complexity exhibited by this class of natural products. This highlight provides an overview of the recent advances in understanding the diversity of these polyketide synthase (PKS) building blocks.

Graphical abstract: Recent advances in the biosynthesis of unusual polyketide synthase substrates
From the themed collection: Biosynthetic Assembly Lines
Highlight

Interrupted adenylation domains: unique bifunctional enzymes involved in nonribosomal peptide biosynthesis

This highlight focuses on one of Nature's key strategies to doubly modify an amino acid during nonribosomal peptide biosynthesis by using a single enzyme, an interrupted adenylation domain.

Graphical abstract: Interrupted adenylation domains: unique bifunctional enzymes involved in nonribosomal peptide biosynthesis
From the themed collection: Biosynthetic Assembly Lines
Review Article

Unique marine derived cyanobacterial biosynthetic genes for chemical diversity

Cyanobacteria are a prolific source of structurally unique and biologically active natural products that derive from intriguing biochemical pathways.

Graphical abstract: Unique marine derived cyanobacterial biosynthetic genes for chemical diversity
From the themed collection: Biosynthetic Assembly Lines
Open Access Review Article

Recent advances in engineering nonribosomal peptide assembly lines

This reviews summarises progress towards the engineering of nonribosomal peptide synthetases (NRPS) from the expression of heterologous tailoring enzymes to direct modifications of the assembly lines to produce analogues. New techniques/tools for introducing changes are also examined.

Graphical abstract: Recent advances in engineering nonribosomal peptide assembly lines
From the themed collection: Biosynthetic Assembly Lines
Review Article

Biosynthesis of polyketides by trans-AT polyketide synthases

This review discusses the biosynthesis of natural products that are generated by trans-AT polyketide synthases, a family of catalytically versatile enzymes that represents one of the major group of proteins involved in the production of bioactive polyketides.

Graphical abstract: Biosynthesis of polyketides by trans-AT polyketide synthases
From the themed collection: Biosynthetic Assembly Lines
Review Article

Genetic engineering of modular PKSs: from combinatorial biosynthesis to synthetic biology

This reviews covers on-going efforts at engineering the gigantic modular polyketide synthases (PKSs), highlighting both notable successes and failures.

Graphical abstract: Genetic engineering of modular PKSs: from combinatorial biosynthesis to synthetic biology
From the themed collection: Biosynthetic Assembly Lines
Review Article

Polyketide synthase and non-ribosomal peptide synthetase thioesterase selectivity: logic gate or a victim of fate?

Thioesterases (TEs) are product offloading enzymes from FAS, PKS, and NRPS complexes. We review the diversity, structure, and mechanism of PKS and NRPS TEs and analyze TE loading and release steps as possible logic gates with a view to predicting TE function in new pathways.

Graphical abstract: Polyketide synthase and non-ribosomal peptide synthetase thioesterase selectivity: logic gate or a victim of fate?
From the themed collection: Biosynthetic Assembly Lines
Review Article

Uncovering the structures of modular polyketide synthases

This review covers a breakthrough in the structural biology of the gigantic modular polyketide synthases (PKS): the structural characterization of intact modules by single-particle cryo-electron microscopy and small-angle X-ray scattering.

Graphical abstract: Uncovering the structures of modular polyketide synthases
From the themed collection: Biosynthetic Assembly Lines
14 articles

About this collection

Natural Product Reports is delighted to present a themed issue dedicated to biosynthetic assembly line. Guest-edited by Professors Greg Challis and Barrie Wilkinson, the collection features contributions from leaders in the field and highlights how the recent advances in understanding the enzymology and structural biology of biosynthetic assembly lines are bringing many of the underlying molecular mechanistic principles into clear view.

Spotlight

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