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Themed collection Strategies for Cellular Target Identification of Natural Products

13 items
Editorial

Editorial: Strategies for cellular target identification of natural products

Daniel Romo and Jun Liu introduce the Natural Product Reports themed issue on ‘Strategies for cellular target identification of natural products’.

Graphical abstract: Editorial: Strategies for cellular target identification of natural products
Highlight

Integrated profiling methods for identifying the targets of bioactive compounds: MorphoBase and ChemProteoBase

Target identification of new bioactive compounds has been achieved by both our direct and indirect approaches. Here, we highlight the utility of the latter approaches, MorphoBase and ChemProteoBase.

Graphical abstract: Integrated profiling methods for identifying the targets of bioactive compounds: MorphoBase and ChemProteoBase
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Identifying the cellular targets of natural products using T7 phage display

A description of the T7 phage biopanning procedure is provided with tips and advice suitable for setup in a chemistry laboratory.

Graphical abstract: Identifying the cellular targets of natural products using T7 phage display
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Natural products-prompted chemical biology: phenotypic screening and a new platform for target identification

This highlight focuses on our recent discoveries and chemical genetics approaches for bioactive microbial metabolites that target cancer cells, the cancer microenvironment, and cell membrane signalling. In addition, the development of two new platforms to identify the cellular targets of these molecules is also discussed.

Graphical abstract: Natural products-prompted chemical biology: phenotypic screening and a new platform for target identification
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Discoveries, target identifications, and biological applications of natural products that inhibit splicing factor 3B subunit 1

The natural products FR901464, pladienolide, and herboxidiene were discovered as activators of reporter gene systems. They were later found to inhibit the spliceosome.

Graphical abstract: Discoveries, target identifications, and biological applications of natural products that inhibit splicing factor 3B subunit 1
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I only have eye for ewe: the discovery of cyclopamine and development of Hedgehog pathway-targeting drugs

Outbreaks of cyclopic lambs during the 1950s led to the discovery of cyclopamine and new anticancer therapies.

Graphical abstract: I only have eye for ewe: the discovery of cyclopamine and development of Hedgehog pathway-targeting drugs
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Target identification of natural products and bioactive compounds using affinity-based probes

Direct capture of drug–target complexes in situ by using affinity-based probes allows target identification of natural products and bioactive compounds, even if the binding is reversible with moderate affinity.

Graphical abstract: Target identification of natural products and bioactive compounds using affinity-based probes
Highlight

Synthesis and mode of action of oligomeric sesquiterpene lactones

In this highlight we describe two case studies from our laboratory, involving the biomimetic syntheses and the biological mechanism elucidation of the bioactive oligomeric sesquiterpenoids, (+)-ainsliadimer A (4) and (−)-ainsliatrimer A (5).

Graphical abstract: Synthesis and mode of action of oligomeric sesquiterpene lactones
Open Access Review Article

Chemical proteomics approaches for identifying the cellular targets of natural products

This review focuses on chemical probes to identify the protein binding partners of natural products in living systems.

Graphical abstract: Chemical proteomics approaches for identifying the cellular targets of natural products
Review Article

Advances in identification and validation of protein targets of natural products without chemical modification

This review focuses on and reports case studies of the latest advances in target protein identification methods for label-free natural products. The integration of newly developed technologies will provide new insights and highlight the value of natural products for use as biological probes and new drug candidates.

Graphical abstract: Advances in identification and validation of protein targets of natural products without chemical modification
Review Article

Photo-cross-linked small-molecule affinity matrix as a tool for target identification of bioactive small molecules

This review describes the status of the photo-cross-linked small-molecule affinity matrix while providing a useful tutorial for academic and industrial chemical biologists who are involved or interested in drug target identification.

Graphical abstract: Photo-cross-linked small-molecule affinity matrix as a tool for target identification of bioactive small molecules
Review Article

Strategies for target identification of antimicrobial natural products

Despite a pervasive decline in natural product research at many pharmaceutical companies over the last two decades, natural products have undeniably been a prolific and unsurpassed source for new lead antibacterial compounds.

Graphical abstract: Strategies for target identification of antimicrobial natural products
Review Article

Target identification by image analysis

Biologically active compounds induce phenotypic changes in target cells, which can be used to predict their modes of action. Such changes were initially detected by a visual inspection of images, while recent studies are based on high content analysis (HCA) methods using automated microscopy and analysis software.

Graphical abstract: Target identification by image analysis
13 items

About this collection

Natural Product Reports is delighted to present a themed issue dedicated to strategies for cellular target identification of natural products.

The incredible information content of bioactive natural products, revealed in their ability to interact in a highly selective manner with often one or just a few cellular targets in the vast proteome and genome of human cells, continues to profoundly impact basic cell biology and drug discovery. Propelled by remarkable advances in synthetic chemistry, analytical chemistry, bioinformatics, and biology (various ‘omics’), the timeframe required for a natural product to be coupled to its cellular target(s) and also for identification of the minimal pharmacophore has continued to decrease. Guest-edited by Professors Daniel Romo and Jun O. Liu this themed issue covers some of the exciting advances being made toward coupling natural products to their cellular receptors that in turn impacts cell biology and drug discovery.

Articles in this web themed issue will be added below as soon as possible after they are published. Please return to this page frequently to see the collection grow in the run up to the final issue publication.

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