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Themed collection Chemical Biology in Molecular BioSystems

116 items - Showing page 1 of 2
Opinion

Understanding the chemically-reactive proteome

The reactivity of amino acid residues in proteins is context-dependent and difficult to predict.

Graphical abstract: Understanding the chemically-reactive proteome
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Open Access Review Article

Peptide-tags for site-specific protein labelling in vitro and in vivo

Peptide-tag based labelling can be achieved by (i) enzymes (ii) recognition of metal ions or small molecules and (iii) peptide–peptide interactions and enables site-specific protein visualization to investigate protein localization and trafficking.

Graphical abstract: Peptide-tags for site-specific protein labelling in vitro and in vivo
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Review Article

The regulatory function of microRNA-1 in arrhythmias

Arrhythmia, the basis of which is cardiomyocyte ion channel abnormalities, poses a serious threat to human health.

Graphical abstract: The regulatory function of microRNA-1 in arrhythmias
From the themed collection: Chemical Biology in Molecular BioSystems
Review Article

tRNA – the golden standard in molecular biology

Transfer RNAs (tRNAs) represent a major class of RNA molecules for which primary, secondary, and tertiary structures are known.

Graphical abstract: tRNA – the golden standard in molecular biology
From the themed collection: Chemical Biology in Molecular BioSystems
Review Article

Persulfides: current knowledge and challenges in chemistry and chemical biology

This review article summarizes known knowledge about both small molecule persulfides and protein persulfides, including their preparation/detection methods, reactions, and biological implications.

Graphical abstract: Persulfides: current knowledge and challenges in chemistry and chemical biology
From the themed collection: Chemical Biology in Molecular BioSystems
Communication

Membrane activity of a supramolecular peptide-based chemotherapeutic enhancer

This work explored the membrane activity of filamentous supramolecular peptides and their great potential as a supramolecular peptide-based chemotherapeutic enhancer.

Graphical abstract: Membrane activity of a supramolecular peptide-based chemotherapeutic enhancer
From the themed collection: Chemical Biology in Molecular BioSystems
Open Access Communication

Unprecedented staining of polar lipids by a luminescent rhenium complex revealed by FTIR microspectroscopy in adipocytes

Localisation of a neutral rhenium(I) tricarbonyl phenanthroline species to regions of high polar lipid concentrations is demonstrated by Fourier transform infrared (FTIR) microspectroscopy.

Graphical abstract: Unprecedented staining of polar lipids by a luminescent rhenium complex revealed by FTIR microspectroscopy in adipocytes
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Communication

A tyrosine-reactive irreversible inhibitor for glutathione S-transferase Pi (GSTP1)

Glutathione S-transferase Pi (GSTP1) mediates cellular defense against reactive electrophiles.

Graphical abstract: A tyrosine-reactive irreversible inhibitor for glutathione S-transferase Pi (GSTP1)
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Communication

Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

SUMO-fused and intein-fused protein expression systems have been combined to prepare a dual-color labeled ubiquitin sensor for detecting UCH-L3's activity.

Graphical abstract: Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Open Access Communication

Sortase-mediated labelling of lipid nanodiscs for cellular tracing

Lipid nanodiscs have broad applications in membrane protein assays, biotechnology and materials science.

Graphical abstract: Sortase-mediated labelling of lipid nanodiscs for cellular tracing
From the themed collection: Chemical Biology in Molecular BioSystems
Communication

Performance of optimized noncanonical amino acid mutagenesis systems in the absence of release factor 1

Unconditional deletion of RF1 in a genomically recoded E. coli enables multisite noncanonical amino acid incorporation by UAG suppression.

Graphical abstract: Performance of optimized noncanonical amino acid mutagenesis systems in the absence of release factor 1
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Open Access Communication

Stabilization of bacterially expressed erythropoietin by single site-specific introduction of short branched PEG chains at naturally occurring glycosylation sites

Erythropoietin (EPO) with single small branched PEG chains installed by the Staudinger-phosphite reaction leads to enhanced solubility, stability and intact in vitro bioactivity.

Graphical abstract: Stabilization of bacterially expressed erythropoietin by single site-specific introduction of short branched PEG chains at naturally occurring glycosylation sites
From the themed collection: Chemical Biology in Molecular BioSystems
Open Access Communication

Molecular basis of 5-hydroxytryptophan synthesis in Saccharomyces cerevisiae

We report for the first time that 5-hydroxytryptophan can be synthesized in Saccharomyces cerevisiae by heterologously expressing prokaryotic phenylalanine 4-hydroxylase or eukaryotic tryptophan 3/5-hydroxylase, together with enhanced synthesis of MH4 or BH4 cofactors.

Graphical abstract: Molecular basis of 5-hydroxytryptophan synthesis in Saccharomyces cerevisiae
From the themed collection: Chemical Biology in Molecular BioSystems
Communication

Zinc(II)-induced control of the internalization of a near-infrared fluorescent probe by live cells

We describe a NIR-fluorescent marker which is efficiently internalized by live cells in the presence exogenous zinc(II) whereas only negligible staining was detected in the absence of zinc(II).

Graphical abstract: Zinc(ii)-induced control of the internalization of a near-infrared fluorescent probe by live cells
From the themed collection: Chemical Biology in Molecular BioSystems
Open Access Communication

Protonation state of F420H2 in the prodrug-activating deazaflavin dependent nitroreductase (Ddn) from Mycobacterium tuberculosis

Theoretical and experimental studies of the deazaflavin dependent nitroreductase (Ddn) enzyme bound cofactor F420 demonstrate that the reduced cofactor is deprotonated in the holoenzyme form.

Graphical abstract: Protonation state of F420H2 in the prodrug-activating deazaflavin dependent nitroreductase (Ddn) from Mycobacterium tuberculosis
From the themed collection: Chemical Biology in Molecular BioSystems
Open Access Communication

The ring residue proline 8 is crucial for the thermal stability of the lasso peptide caulosegnin II

Lasso peptides are fascinating natural products with a unique structural fold that can exhibit tremendous thermal stability.

Graphical abstract: The ring residue proline 8 is crucial for the thermal stability of the lasso peptide caulosegnin II
From the themed collection: Chemical Biology in Molecular BioSystems
Open Access Communication

Eliminating caspase-7 and cathepsin B cross-reactivity on fluorogenic caspase-3 substrates

Fluorogenic substrates incorporating the sequence Asp-Glu-Pro-Asp-Ser were able to quantify caspase-3 activity without notable caspase-7 and cathepsin B cross-reactivity.

Graphical abstract: Eliminating caspase-7 and cathepsin B cross-reactivity on fluorogenic caspase-3 substrates
From the themed collection: Chemical Biology in Molecular BioSystems
Communication

Signalling to the nucleus under the control of light and small molecules

One major regulatory mechanism in cell signalling is the spatio-temporal control of the localization of signalling molecules. We synthetically designed an entire cell signalling pathway, which allows controlling the transport of signalling molecules from the plasma membrane to the nucleus, by using light and small molecules.

Graphical abstract: Signalling to the nucleus under the control of light and small molecules
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Communication

Reverse Watson–Crick G–G base pair in G-quadruplex formation

Cisplatin binds to N7 of guanine in a reverse Watson–Crick G–G pair.

Graphical abstract: Reverse Watson–Crick G–G base pair in G-quadruplex formation
From the themed collection: Chemical Biology in Molecular BioSystems
Communication

Oligomerization enhancement and two domain swapping mode detection for thermostable cytochrome c552via the elongation of the major hinge loop

High-order oligomers increased whereas N-terminal domain swapping and C-terminal domain swapping were elucidated by the insertion of Gly residues at the major hinge loop of cytochrome c552.

Graphical abstract: Oligomerization enhancement and two domain swapping mode detection for thermostable cytochrome c552via the elongation of the major hinge loop
From the themed collection: Chemical Biology in Molecular BioSystems
Communication

Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents

Direct visualization of bioorthogonal alkyne or azide handles using fluorogenic azide–alkyne cycloaddition conducted on the surface of a blot membrane.

Graphical abstract: Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents
From the themed collection: Chemical Biology in Molecular BioSystems
Communication

A library approach to rapidly discover photoaffinity probes of the mRNA decapping scavenger enzyme DcpS

A photoaffinity library expedited the discovery of a site-specific DcpS probe.

Graphical abstract: A library approach to rapidly discover photoaffinity probes of the mRNA decapping scavenger enzyme DcpS
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Developmental exposure to paraquat and maneb can impair cognition, learning and memory in Sprague-Dawley rats

PQ–MB may affect the behavior and cognitive function of rat offspring through the cAMP–PKA–CREB signaling pathway.

Graphical abstract: Developmental exposure to paraquat and maneb can impair cognition, learning and memory in Sprague-Dawley rats
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Assessment of DNA-binding affinity of cholinesterase reactivators and electrophoretic determination of their effect on topoisomerase I and II activity

In this paper, we describe the biochemical properties and biological activity of a series of cholinesterase reactivators (symmetrical bisquaternary xylene-linked compounds, K106–K114) with ctDNA.

Graphical abstract: Assessment of DNA-binding affinity of cholinesterase reactivators and electrophoretic determination of their effect on topoisomerase I and II activity
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Unfolding knots by proteasome-like systems: simulations of the behaviour of folded and neurotoxic proteins

Knots in proteins have been proposed to resist proteasomal degradation, thought in turn to be related to neurodegenerative diseases such as Huntington.

Graphical abstract: Unfolding knots by proteasome-like systems: simulations of the behaviour of folded and neurotoxic proteins
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

In-gel activity-based protein profiling of a clickable covalent ERK1/2 inhibitor

The trans-cyclooctenol/tetrazine click reaction was used for in-gel ABPP to determine the proteome-wide selectivity profile of a covalent ERK1/2 inhibitor.

Graphical abstract: In-gel activity-based protein profiling of a clickable covalent ERK1/2 inhibitor
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Protection of human γB-crystallin from UV-induced damage by epigallocatechin gallate: spectroscopic and docking studies

The transparency of the human eye lens depends on the solubility and stability of the structural proteins of the eye lens, the crystallins.

Graphical abstract: Protection of human γB-crystallin from UV-induced damage by epigallocatechin gallate: spectroscopic and docking studies
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Supramolecular guest relay using host-protein nanocavities: an application of host-induced guest protonation

Small drug molecules and other important metabolites are delivered via a suitable carrier protein-mediated transport through a specific receptor.

Graphical abstract: Supramolecular guest relay using host-protein nanocavities: an application of host-induced guest protonation
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

SOHPRED: a new bioinformatics tool for the characterization and prediction of human S-sulfenylation sites

SOHPRED is a new and competitive bioinformatics tool for characterizing and predicting human S-sulfenylation sites.

Graphical abstract: SOHPRED: a new bioinformatics tool for the characterization and prediction of human S-sulfenylation sites
From the themed collection: Chemical Biology in Molecular BioSystems
Open Access Paper

Role of the transmembrane domain in SNARE protein mediated membrane fusion: peptide nucleic acid/peptide model systems

Analogs of the Soluble NSF (N-ethylmaleimide-sensitive factor) Attachment Protein Receptor proteins (SNAREs) for mediation of vesicle fusion.

Graphical abstract: Role of the transmembrane domain in SNARE protein mediated membrane fusion: peptide nucleic acid/peptide model systems
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Cell cytotoxicity and serum albumin binding capacity of the morin–Cu(II) complex and its effect on deoxyribonucleic acid

Oxidative DNA damage is confirmed by the morin–Cu(II) complex and it is also able to inhibit the growth of human HeLa cells. The binding of the morin–Cu(II) complex with HSA and BSA occurs mainly through hydrophobic forces.

Graphical abstract: Cell cytotoxicity and serum albumin binding capacity of the morin–Cu(ii) complex and its effect on deoxyribonucleic acid
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Virucidal activity of human α- and β-defensins against hepatitis C virus genotype 4

We revealed that human α- and β-defensins have strong anti-HCV activity in experiments on cellular protection, neutralization, and treatment at low concentrations, whereas synthetic linear avian defensins could reach similar anti-HCV potential only at noticeably higher concentrations.

Graphical abstract: Virucidal activity of human α- and β-defensins against hepatitis C virus genotype 4
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

Molecular dynamics simulations provide insights into the substrate specificity of FAOX family members

Enzymatic assays based on Fructosyl Amino Acid Oxidases (FAOX) represent a potential, rapid and economical strategy to measure glycated hemoglobin (HbA1c), which is in turn a reliable method to monitor the insurgence and the development of diabetes mellitus.

Graphical abstract: Molecular dynamics simulations provide insights into the substrate specificity of FAOX family members
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Histidine switch controlling pH-dependent protein folding and DNA binding in a transcription factor at the core of synthetic network devices

Reversible protonation of histidine at the dimer interface of HucR controls interconversion between molten globule and compact folded state.

Graphical abstract: Histidine switch controlling pH-dependent protein folding and DNA binding in a transcription factor at the core of synthetic network devices
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

Analyzing ERK 1/2 signalling and targets

The ERK cascade (e.g. Raf-1) protects the heart from cell death and ischemic injury but can also turn maladaptive.

Graphical abstract: Analyzing ERK 1/2 signalling and targets
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

Discovery of a potent cyclooxygenase-2 inhibitor, S4, through docking-based pharmacophore screening, in vivo and in vitro estimations

Cyclooxygenase (COX; EC: 1.14.99.1), the key enzyme in prostaglandin production in the human body, is a major pharmacological target for developing anti-inflammatory agents.

Graphical abstract: Discovery of a potent cyclooxygenase-2 inhibitor, S4, through docking-based pharmacophore screening, in vivo and in vitro estimations
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

The long noncoding RNA HOTAIR activates autophagy by upregulating ATG3 and ATG7 in hepatocellular carcinoma

The long noncoding RNA HOX transcript antisense RNA (HOTAIR) has been reported to be an oncogene that influences tumor cell development and that correlates with prognosis in hepatocellular carcinoma (HCC).

Graphical abstract: The long noncoding RNA HOTAIR activates autophagy by upregulating ATG3 and ATG7 in hepatocellular carcinoma
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Myricetin arrests human telomeric G-quadruplex structure: a new mechanistic approach as an anticancer agent

The use of small molecules to arrest G-quadruplex structure has become a potential strategy for the development and design of a new class of anticancer therapeutics.

Graphical abstract: Myricetin arrests human telomeric G-quadruplex structure: a new mechanistic approach as an anticancer agent
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

The unique functional role of the C–H⋯S hydrogen bond in the substrate specificity and enzyme catalysis of type 1 methionine aminopeptidase

Unique C–H⋯S hydrogen bonding interactions allow nature to attain recognition specificity between molecular interfaces where there is no apparent scope for classical hydrogen bonding or polar interactions.

Graphical abstract: The unique functional role of the C–H⋯S hydrogen bond in the substrate specificity and enzyme catalysis of type 1 methionine aminopeptidase
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

A clickable glutathione approach for identification of protein glutathionylation in response to glucose metabolism

Clickable glutathione was used for analyzing the reversible change of protein glutathionylation in response to glucose metabolism and mitochondrial ROS.

Graphical abstract: A clickable glutathione approach for identification of protein glutathionylation in response to glucose metabolism
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Screening dietary flavonoids for the reversal of P-glycoprotein-mediated multidrug resistance in cancer

P-Glycoprotein (P-gp) serves as a therapeutic target for the development of inhibitors to overcome multidrug resistance in cancer cells.

Graphical abstract: Screening dietary flavonoids for the reversal of P-glycoprotein-mediated multidrug resistance in cancer
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Base excision DNA repair in the embryonic development of the sea urchin, Strongylocentrotus intermedius

We have characterized the profile of several key base excision repair activities in the developing embryo of the grey sea urchin, Strongylocentrotus intermedius, at several stages of development.

Graphical abstract: Base excision DNA repair in the embryonic development of the sea urchin, Strongylocentrotus intermedius
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Structural analysis of oncogenic mutation of isocitrate dehydrogenase 1

Arginine to histidine mutation at position 132 (R132H) in isocitrate dehydrogenase 1 (IDH1) led to reduced affinity of the respective enzymes for isocitrate and increased affinity for α-ketoglutarate (AKG) and NADPH.

Graphical abstract: Structural analysis of oncogenic mutation of isocitrate dehydrogenase 1
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Fc-based delivery system enhances immunogenicity of a tuberculosis subunit vaccine candidate consisting of the ESAT-6:CFP-10 complex

Tuberculosis (TB) remains a major global health threat despite chemotherapy and Bacilli Calmette–Guérin (BCG) vaccination.

Graphical abstract: Fc-based delivery system enhances immunogenicity of a tuberculosis subunit vaccine candidate consisting of the ESAT-6:CFP-10 complex
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Elucidation, functional clustering and structural characterization of βTrCP1 substrates through a molecular dynamics study

Structural knowledge of substrate recognition by SKP1–CUL1–βTrCP1 complex for targeted cancer therapeutic strategy.

Graphical abstract: Elucidation, functional clustering and structural characterization of βTrCP1 substrates through a molecular dynamics study
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

NTSMDA: prediction of miRNA–disease associations by integrating network topological similarity

Recently, accumulating studies have indicated that microRNAs (miRNAs) play an important role in exploring the pathogenesis of various human diseases at the molecular level and may result in the design of specific tools for diagnosis, treatment evaluation and prevention.

Graphical abstract: NTSMDA: prediction of miRNA–disease associations by integrating network topological similarity
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Systematic network-based discovery of a Fam20C inhibitor (FL-1607) with apoptosis modulation in triple-negative breast cancer

Family with sequence similarity 20, member C (Fam20C) is a physiological Golgi casein kinase that phosphorylates multiple secreted proteins.

Graphical abstract: Systematic network-based discovery of a Fam20C inhibitor (FL-1607) with apoptosis modulation in triple-negative breast cancer
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

Autophagy is involved in regulating VEGF during high-glucose-induced podocyte injury

Podocytes are the major sites of vascular endothelial growth factor (VEGF) production in kidneys.

Graphical abstract: Autophagy is involved in regulating VEGF during high-glucose-induced podocyte injury
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

TMEM45B promotes proliferation, invasion and migration and inhibits apoptosis in pancreatic cancer cells

Silencing of TMEM45B induces apoptosis and suppresses invasion and migration in pancreatic cancer cells.

Graphical abstract: TMEM45B promotes proliferation, invasion and migration and inhibits apoptosis in pancreatic cancer cells
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

Photo-crosslinking of clinically relevant kinases using H89-derived photo-affinity probes

H89-derived photo-affinity probes can be used to label clinically relevant kinases as well as to screen known and identify novel kinase inhibitors.

Graphical abstract: Photo-crosslinking of clinically relevant kinases using H89-derived photo-affinity probes
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Behaviour of oleic acid-depleted bovine alpha-lactalbumin made LEthal to tumor cells (BAMLET)

Oleic acid (OA) complexes of human alpha-lactalbumin (α-LA) and several other proteins are effective in the killing of a variety of tumor cells.

Graphical abstract: Behaviour of oleic acid-depleted bovine alpha-lactalbumin made LEthal to tumor cells (BAMLET)
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Correlating Flavivirus virulence and levels of intrinsic disorder in shell proteins: protective roles vs. immune evasion

Computational analyses revealed correlations between the intrinsic disorder propensity of shell proteins and case fatality rates (CFRs) among Flaviviruses and within at least two Flavivirus species, such as tick-borne encephalitis virus (TBEV) and dengue virus (DENV).

Graphical abstract: Correlating Flavivirus virulence and levels of intrinsic disorder in shell proteins: protective roles vs. immune evasion
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Protein DJ-1 and its anti-oxidative stress function play an important role in renal cell mediated response to profibrotic agents

In the pathogenesis of renal fibrosis, oxidative stress (OS) enhances the production of reactive oxygen species (ROS) leading to sustained cell growth, inflammation, excessive tissue remodelling and accumulation, which results in the development and acceleration of renal damage.

Graphical abstract: Protein DJ-1 and its anti-oxidative stress function play an important role in renal cell mediated response to profibrotic agents
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Translesion synthesis past guanine(C8)–thymine(N3) intrastrand cross-links catalyzed by selected A- and Y-family polymerases

Guanine(C8)–thymine(N3) intrastrand cross-links are bypassed with varying efficiencies by Y-family polymerases, but the A-family polymerase BF is strongly blocked.

Graphical abstract: Translesion synthesis past guanine(C8)–thymine(N3) intrastrand cross-links catalyzed by selected A- and Y-family polymerases
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Design and development of histone deacetylase (HDAC) chemical probes for cell-based profiling

Validation of novel chemical probes containing photoreactive crosslinking groups and biotin or click reporters is reported for the proteomic study of HDAC complexes.

Graphical abstract: Design and development of histone deacetylase (HDAC) chemical probes for cell-based profiling
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

Docking and molecular dynamics simulation study of EGFR1 with EGF-like peptides to understand molecular interactions

Epidermal growth factor receptors (EGFRs) are oncogenes, which regulate the expression of genes in various pathways, allowing cells to grow and divide.

Graphical abstract: Docking and molecular dynamics simulation study of EGFR1 with EGF-like peptides to understand molecular interactions
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition

Over-expression of the mesenchymal transcription factor Snail alters expression of many proteins. These changes are largely correlated with changes in S-palmitoylation, but in some cases are uncoupled.

Graphical abstract: Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Benzimidazole covalent probes and the gastric H+/K+-ATPase as a model system for protein labeling in a copper-free setting

Synthesis and use of benzimidazole activity-based probes to validate target labeling and identify novel binding partners.

Graphical abstract: Benzimidazole covalent probes and the gastric H+/K+-ATPase as a model system for protein labeling in a copper-free setting
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

An immunochemical approach to detect oxidized protein tyrosine phosphatases using a selective C-nucleophile tag

We report a simplified immunochemical approach to directly detect and quantify oxidized protein tyrosine phosphatases modified with dimedone.

Graphical abstract: An immunochemical approach to detect oxidized protein tyrosine phosphatases using a selective C-nucleophile tag
From the themed collection: Chemical Biology in Molecular BioSystems
Open Access Paper

Effects of mono- and divalent metal ions on DNA binding and catalysis of human apurinic/apyrimidinic endonuclease 1

Here, we used stopped-flow fluorescence techniques to conduct a comparative kinetic analysis of the conformational transitions in human apurinic/apyrimidinic endonuclease 1 (APE1) and in DNA containing an abasic site in the course of their interaction.

Graphical abstract: Effects of mono- and divalent metal ions on DNA binding and catalysis of human apurinic/apyrimidinic endonuclease 1
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

DNA damaging, cell cytotoxicity and serum albumin binding efficacy of the rutin–Cu(II) complex

The rutin–Cu(II) complex causes DNA damage and is also able to inhibit the growth of human HeLa cells. This complex binds with serum albumins via hydrophobic forces.

Graphical abstract: DNA damaging, cell cytotoxicity and serum albumin binding efficacy of the rutin–Cu(ii) complex
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

[Ru(bpy)2(7-CH3-dppz)]2+ and [Ru(phen)2(7-CH3-dppz)]2+ as metallointercalators that affect third-strand stabilization of the poly(U)˙poly(A)*poly(U) triplex

Ru1 and Ru2 not only can stabilize the triplex, but also can serve as molecular “light switches” for the triplex. In addition, the two complexes stabilizing third-strand is weaker, reflecting the binding of Ru1 and Ru2 with the triplex is favored by the Watson–Crick base-paired duplex.

Graphical abstract: [Ru(bpy)2(7-CH3-dppz)]2+ and [Ru(phen)2(7-CH3-dppz)]2+ as metallointercalators that affect third-strand stabilization of the poly(U)˙poly(A)*poly(U) triplex
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

Furanodiene alters mitochondrial function in doxorubicin-resistant MCF-7 human breast cancer cells in an AMPK-dependent manner

Furanodiene is a bioactive sesquiterpene isolated from the spice-producing Curcuma wenyujin plant (Y. H. Chen and C. Ling) (C. wenyujin), which is a commonly prescribed herb used in clinical cancer therapy by modern practitioners of traditional Chinese medicine.

Graphical abstract: Furanodiene alters mitochondrial function in doxorubicin-resistant MCF-7 human breast cancer cells in an AMPK-dependent manner
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Silencing of USP22 suppresses high glucose-induced apoptosis, ROS production and inflammation in podocytes

Silencing of USP22 suppresses high glucose-induced podocyte injury.

Graphical abstract: Silencing of USP22 suppresses high glucose-induced apoptosis, ROS production and inflammation in podocytes
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

Exploring the gating mechanisms of aquaporin-3: new clues for the design of inhibitors?

The pH gating of human AQP3 and its effects on both water and glycerol permeabilities have been fully characterized for the first time using a human red blood cell model (hRBC).

Graphical abstract: Exploring the gating mechanisms of aquaporin-3: new clues for the design of inhibitors?
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

The binding landscape of plasmepsin V and the implications for flap dynamics

Plasmepsin V belongs to the plasmepsin family of aspartic proteases.

Graphical abstract: The binding landscape of plasmepsin V and the implications for flap dynamics
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

Identification of novel inhibitors of DDR1 against idiopathic pulmonary fibrosis by integrative transcriptome meta-analysis, computational and experimental screening

This study reveals DDR1 plays an important role in idiopathic pulmonary fibrosis and identifies a DDR1 inhibitor for possible future therapy.

Graphical abstract: Identification of novel inhibitors of DDR1 against idiopathic pulmonary fibrosis by integrative transcriptome meta-analysis, computational and experimental screening
From the themed collection: Chemical Biology in Molecular BioSystems
Paper

Post-translational control of protein function with light using a LOV-intein fusion protein

Modified split-inteins are fused to the photosensitive LOV2 domain to enable photoactivated cis intein splicing.

Graphical abstract: Post-translational control of protein function with light using a LOV-intein fusion protein
From the themed collection: Chemical Biology in Molecular BioSystems
Open Access Paper

3-Ketosphinganine provokes the accumulation of dihydroshingolipids and induces autophagy in cancer cells

R = H, acyl; X = H, D; Y = H, phosphate, phosphocholine, glucose, or lactose.

Graphical abstract: 3-Ketosphinganine provokes the accumulation of dihydroshingolipids and induces autophagy in cancer cells
From the themed collection: 2016 Hot Articles in Molecular BioSystems
Paper

Probing fatty acid metabolism in bacteria, cyanobacteria, green microalgae and diatoms with natural and unnatural fatty acids

In both eukaryotes and prokaryotes, fatty acid synthases are responsible for the biosynthesis of fatty acids in an iterative process, extending the fatty acid by two carbon units every cycle.

Graphical abstract: Probing fatty acid metabolism in bacteria, cyanobacteria, green microalgae and diatoms with natural and unnatural fatty acids
From the themed collection: Chemical Biology in Molecular BioSystems
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About this collection

Molecular BioSystems publishes many excellent works covering Chemical Biology research, including both chemical tools for biological questions, and molecular-level understanding, control and manipulation of biological processes. (Please read our full scope for further information)

This collection will help your find these articles in a single click, for a quick access to research that matters to you. Updated regularly, for a current look at this important topic area.

A short link for the collection is: http://rsc.li/mb-chemical-biology

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