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Themed collection Biological optical imaging

22 items
Feature Article

Molecular imaging probes for multi-spectral optoacoustic tomography

In this review, we discuss recent progress in emerging optoacoustic probes, their mechanisms, applications and challenges for biological imaging using MSOT.

Graphical abstract: Molecular imaging probes for multi-spectral optoacoustic tomography
From the themed collection: Biological optical imaging
Feature Article

Imaging and therapeutic applications of zinc(II)-dipicolylamine molecular probes for anionic biomembranes

Synthetic ZnDPA receptors are used for molecular imaging of disease and targeted therapeutics.

Graphical abstract: Imaging and therapeutic applications of zinc(ii)-dipicolylamine molecular probes for anionic biomembranes
From the themed collection: Biological optical imaging
Communication

Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety.

Graphical abstract: Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity
From the themed collection: Biological optical imaging
Communication

A lysosome targetable fluorescent probe for endogenous imaging of hydrogen peroxide in living cells

A lysosome targetable naphthalimide based fluorescent probe has been designed and synthesized for selective imaging of H2O2 in different cells, tissues and in vivo models.

Graphical abstract: A lysosome targetable fluorescent probe for endogenous imaging of hydrogen peroxide in living cells
From the themed collection: Biological optical imaging
Communication

Enzyme-responsive reporter molecules for selective localization and fluorescence imaging of pathogenic biofilms

A novel enzyme-responsive reporter molecule (ERM-1) for selective localization of AmpC in pathogenic biofilms.

Graphical abstract: Enzyme-responsive reporter molecules for selective localization and fluorescence imaging of pathogenic biofilms
From the themed collection: Biological optical imaging
Open Access Communication

Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids

Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor.

Graphical abstract: Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids
From the themed collection: Biological optical imaging
Communication

Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells.

Graphical abstract: Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen
From the themed collection: Biological optical imaging
Communication

moxDendra2: an inert photoswitchable protein for oxidizing environments

The authors describe the engineering of the first monomeric photoswitchable fluorescent protein for use in oxidizing cellular enviornments.

Graphical abstract: moxDendra2: an inert photoswitchable protein for oxidizing environments
From the themed collection: Biological optical imaging
Communication

Affibody-targeted fluorogen activating protein for in vivo tumor imaging

Compact molecular imaging reagents made of affibody-fused fluorogen activating proteins enable high-contrast imaging or pre-targeting and rapid fluorescent detection.

Graphical abstract: Affibody-targeted fluorogen activating protein for in vivo tumor imaging
From the themed collection: Biological optical imaging
Communication

PSMA-targeted contrast agents for intraoperative imaging of prostate cancer,

Net charges, spacer length, hydrophobicity, and polarity effects on PSMA-targeted NIR fluorophores.

Graphical abstract: PSMA-targeted contrast agents for intraoperative imaging of prostate cancer,
From the themed collection: Biological optical imaging
Communication

A bioorthogonal ‘turn-on’ fluorescent probe for tracking mitochondrial nitroxyl formation

A bioorthogonal mitochondria targeted HNO sensor was applied for the detection of biological concentrations of HNO in vitro in organelles.

Graphical abstract: A bioorthogonal ‘turn-on’ fluorescent probe for tracking mitochondrial nitroxyl formation
From the themed collection: Biological optical imaging
Communication

Development of a BODIPY-based fluorescent probe for imaging pathological tau aggregates in live cells

As a cell-permeable imaging probe, BD-tau selectively labeled pathological tau aggregates in live neuronal cells.

Graphical abstract: Development of a BODIPY-based fluorescent probe for imaging pathological tau aggregates in live cells
From the themed collection: Biological optical imaging
Communication

Targeted combinational therapy inducing mitochondrial dysfunction

Mitochondria-directed GSH-activated release of a chlorambucil derivative and an AIE dye induces significantly increased mitochondrial dysfunction and apoptosis.

Graphical abstract: Targeted combinational therapy inducing mitochondrial dysfunction
From the themed collection: Biological optical imaging
Communication

A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy

We report an advanced monomeric far-red fluorescent marker protein, mGarnet2, which shows excellent performance as a live-cell fusion marker for microscopy as well as STED nanoscopy with 640 nm excitation and 780 nm depletion wavelengths.

Graphical abstract: A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy
From the themed collection: Biological optical imaging
Communication

Intrinsic blinking of red fluorescent proteins for super-resolution microscopy

Blinking of some RFPs in live untreated cells can be used to reconstruct super-resolved images.

Graphical abstract: Intrinsic blinking of red fluorescent proteins for super-resolution microscopy
From the themed collection: Biological optical imaging
Communication

A Trp-BODIPY cyclic peptide for fluorescence labelling of apoptotic bodies

We describe the synthesis and characterization of a Trp-BODIPY fluorogenic peptide for labelling subcellular bodies released by human apoptotic cells.

Graphical abstract: A Trp-BODIPY cyclic peptide for fluorescence labelling of apoptotic bodies
From the themed collection: Biological optical imaging
Communication

An efficient two-photon fluorescent probe for human NAD(P)H:quinone oxidoreductase (hNQO1) detection and imaging in tumor cells

The probe TPQ, which displays high selectivity and anti-interference ability, was successfully applied to endogenous hNQO1 imaging and for the identification of different cancer cells.

Graphical abstract: An efficient two-photon fluorescent probe for human NAD(P)H:quinone oxidoreductase (hNQO1) detection and imaging in tumor cells
From the themed collection: Biological optical imaging
Communication

Headgroup engineering in mechanosensitive membrane probes

Tricky chemistry had to be addressed to make mechanosensitive membrane probes ready for use, including a chalcogen-bond mediated “molecular guillotinylation”.

Graphical abstract: Headgroup engineering in mechanosensitive membrane probes
From the themed collection: Biological optical imaging
Open Access Communication

Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes

We have explored the potential utility of several new designs for genetically encoded indicators of membrane potential.

Graphical abstract: Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes
From the themed collection: Biological optical imaging
Communication

Real-time monitoring of vesicle pH in an endocytic pathway using an EGF-conjugated two-photon probe

An EGF-conjugated two-photon probe for real-time monitoring and quantitative analysis of luminal pH values during endocytic pathway activity was reported.

Graphical abstract: Real-time monitoring of vesicle pH in an endocytic pathway using an EGF-conjugated two-photon probe
From the themed collection: Biological optical imaging
Open Access Communication

Green-to-red primed conversion of Dendra2 using blue and red lasers

Primed photoconversion of Dendra2 in commonly available confocal and super-resolution microscopy setups equipped with blue and red lasers is described.

Graphical abstract: Green-to-red primed conversion of Dendra2 using blue and red lasers
From the themed collection: Biological optical imaging
Research Article

A histone H1-binding-aptide-based apoptosis imaging probe for monitoring tumor responses to cancer therapy

Here we report the feasibility of using a high-affinity histone H1-binding peptide as a potential molecular apoptosis imaging probe.

Graphical abstract: A histone H1-binding-aptide-based apoptosis imaging probe for monitoring tumor responses to cancer therapy
From the themed collection: Biological optical imaging
22 items

About this collection

This web themed collection, guest edited by Robert Campbell (University of Alberta), Carsten Schultz (EMBL) and Young-Tae Chang (National University of Singapore), is a celebration of current achievements and future perspectives in the field of Biological Optical Imaging. The last decade has seen tremendous advances in methods for optical imaging and sensing in live cells. These practical advances have come as a combination of sudden leaps forward and steady development of improved versions of existing tools. This web themed collection aims to provide a sampling of the full landscape of biological optical imaging research, ranging from fundamental photophysical studies, to methodology and techniques (both molecular and instrumental), to biological applications of optical imaging.

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