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Chapter 24

Using Microfluidics, Real-time Imaging and Mathematical Modelling to study GPCR Signalling

Multiple detailed mechanisms have been proposed to describe GPCR-mediated intracellular calcium oscillations and have been formulated as mathematical models. Here we describe phase-locking analysis, a qualitative test that can be used to evaluate those models. Phase-locking refers to the phenomenon whereby an oscillatory system synchronizes to a periodic input. In our experiments, single cells are subjected to square-wave pulses of ligand in a microfluidic device and calcium is imaged using the FRET probe YC3.60. Comparison of cell responses with model predictions gives insights into relevant biological mechanisms. We anticipate that phase-locking analysis will be useful in evaluating GPCR as well as other signalling pathways.

Print publication date: 30 Aug 2011
Copyright year: 2011
Print ISBN: 978-1-84973-183-6
PDF eISBN: 978-1-84973-344-1
From the book series:
RSC Drug Discovery