Issue 14, 2024
From the journal:

Chemical Communications

Single-molecule detection assisted by the target-triggered signal amplification strategy for ultrasensitive quantitative analysis of intracellular telomerase activity†

Chengfang Jiang,a   Qi Wang,a   Jing Geng,b   Mengmeng Li,a   Yuqi Zhang,a   Xinli Shi,a   Yan Zhang,a   Xinyue Song ORCID logo *b  and  Shusheng Zhang ORCID logo *b  

* Corresponding authors

a College of Chemistry and Chemical Engineering, Linyi University, P. R. China

b Linyi Mental Health Center, Linyi City, Shandong Province, P.R. China
E-mail: songxinyue428@163.com, shushzhang@126.com

Abstract

We developed a multiplex single-molecule quantitative assay of intracellular telomerase that used target-triggered signal amplification to enhance sensitivity, substrate reaction to increase signal stability, and quantum dots to enhance signal-to-noise ratio, obtaining an LOD of 5 × 10−14 IU for intracellular telomerase and LOD of 3 cells for multiple cancer cells.

Graphical abstract: Single-molecule detection assisted by the target-triggered signal amplification strategy for ultrasensitive quantitative analysis of intracellular telomerase activity

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/D3CC05683J
Article type
Communication
Submitted
20 Nov 2023
Accepted
29 Dec 2023
First published
09 Jan 2024

Chem. Commun., 2024,60, 1912-1915

Permissions

Request permissions

Single-molecule detection assisted by the target-triggered signal amplification strategy for ultrasensitive quantitative analysis of intracellular telomerase activity

C. Jiang, Q. Wang, J. Geng, M. Li, Y. Zhang, X. Shi, Y. Zhang, X. Song and S. Zhang, Chem. Commun., 2024, 60, 1912 DOI: 10.1039/D3CC05683J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements