Temperature dependence of the viscoelastic properties of a natural gastropod mucus by Brillouin light scattering spectroscopy

Abstract

Brillouin spectroscopy was used to probe the viscoelastic properties of a natural gastropod mucus at GHz frequencies over the range −11 °C ≤ T ≤ 52 °C. Anomalies in the temperature dependence of mucus longitudinal acoustic mode peak parameters and associated viscoelastic properties at T = −2.5 °C, together with the appearance of a peak due to ice at this temperature, suggest that the mucus undergoes a phase transition from a viscous liquid state to one in which liquid mucus and solid ice phases coexist. Failure of this transition to proceed to completion even at −11 °C is attributed to glycoprotein–water interaction. The temperature dependence of the viscoelastic properties and the phase behaviour suggest that water molecules bind to glycoprotein at a temperature above the onset of freezing and that the reduced ability of this bound water to take on a configuration that facilitates freezing is responsible for the observed freezing point depression and gradual nature of the liquid–solid transition.