A colloidal gold immunochromatographic strip assay for the rapid detection of Shigella in milk and meat products

Abstract

Shigella is the most common foodborne pathogen and can cause bacillary dysentery. Therefore, it is necessary to detect Shigella rapidly and accurately, but it is difficult for traditional methods to meet these requirements. In this study, monoclonal antibodies (mAbs) were prepared using a recombinant invasive plasmid antigen C (IpaC) protein, which is specifically located on the outer membrane of Shigella. Based on our mAbs 2G11 and 5A11, a colloidal gold lateral flow immunochromatographic (LFIA) test strip method for the detection of several serotypes of Shigella was produced. The sensitivity of the LFIA strip was 1 × 10⁵ CFU mL⁻¹ for four groups, and seven serotypes of Shigella. The applicability of the strip was tested using spiked milk and meat samples and the results showed that Shigella could be detected in these matrices.